Nanoparticle System for High Throughput Proteomics

用于高通量蛋白质组学的纳米颗粒系统

• 批准号: 6881871
• 负责人: CYNTHIA C. BAMDAD
• 金额: $ 19.9万
• 依托单位: MINERVA BIOTECHNOLOGIES CORPORATION
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-05-01 至 2007-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6881871
• 关键词: Nanoparticle; System; Throughput; Proteomics

DESCRIPTION (provided by applicant): Minerva Biotechnologies is a nanoparticle company that has developed proprietary technology that enables high throughput proteomics. Without the development of new enabling technologies, sequential testing of every possible pair-wise interaction would require about a billion experiments. However, assuming about 40,000 proteins in the proteome and each might interact with five real binding partners, we really only need to identify 160,000 interacting pairs. We reasoned that if we could stably attach a protein to its encoding DMA, and if we could isolate interacting partners from non-interacting proteins, the problem of generating a proteome-wide 3-dimensional interaction map would be soluble. The proposal under review combines the power of yeast 2-hybrid with the accuracy of in vitro testing. This proposal describes a complete nanotechnology system that will enable the mapping of large protein interaction networks in massively parallel format.

描述（由申请人提供）：Minerva Biotechnologies 是一家纳米粒子公司，开发了可实现高通量蛋白质组学的专有技术。如果不开发新的支持技术，对每一种可能的成对相互作用的顺序测试将需要大约十亿次实验。然而，假设蛋白质组中有大约 40,000 个蛋白质，并且每个蛋白质都可能与 5 个真实的结合伙伴相互作用，我们实际上只需要识别 160,000 个相互作用对。我们推断，如果我们能够将蛋白质稳定地附着到其编码 DMA 上，并且如果我们能够将相互作用的伙伴与非相互作用的蛋白质分离，那么生成蛋白质组范围的 3 维相互作用图谱的问题将得到解决。正在审查的提案将酵母 2-hybrid 的强大功能与体外测试的准确性结合起来。该提案描述了一个完整的纳米技术系统，它将能够以大规模并行格式绘制大型蛋白质相互作用网络。