The Role of Argonaute in RNAi

Argonaute 在 RNAi 中的作用

• 批准号: 6969860
• 负责人: Leemor Joshua-Tor
• 金额: $ 31.7万
• 依托单位: COLD SPRING HARBOR LABORATORY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-01 至 2009-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6969860
• 关键词: RNA interference; active sites; conformation; crosslink; crystallization; enzyme activity; gene induction /repression; heat shock proteins; nuclease; protein purification; protein quantitation /detection; protein structure function; small interfering RNA

DESCRIPTION (provided by applicant): RNA interference (RNAi) has taken experimental biology by storm in recent years, since it has been greatly exploited as an ever increasingly effective tool to study gene function by gene silencing through RNAi. It is also being developed as a possible therapeutic tool. The RNAi machinery has been shown to have a role in development and appears to be important in maintaining stem cell identity. Though there has been remarkable progress in unraveling the components of the RNAi machinery, we are just beginning to understand how they work at the molecular level. The objective of this proposal is to provide the structural and biochemical framework, which will enable us to understand the mechanism of gene silencing by RNAi, with a focus on the signature component of the RNA-induced silencing complex (RISC), the Argonaute protein.

描述（由申请人提供）：近年来，RNA干扰（RNAi）席卷了实验生物学，因为它已被广泛用作通过RNAi沉默基因来研究基因功能的日益有效的工具。它也被开发为一种可能的治疗工具。 RNAi 机制已被证明在发育中发挥作用，并且对于维持干细胞身份似乎很重要。尽管在解开 RNAi 机制的组成部分方面已经取得了显着的进展，但我们才刚刚开始了解它们在分子水平上的工作原理。该提案的目的是提供结构和生化框架，使我们能够了解 RNAi 基因沉默的机制，重点关注 RNA 诱导沉默复合物 (RISC) 的标志性成分——Argonaute 蛋白。