Mutualism between X. nematophila and S. carpocapsae

X. nematophila 和 S. carpocapsae 之间的互利共生

• 批准号: 6931509
• 负责人: ARCHNA BHASIN
• 金额: $ 4.83万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-08-01 至 2006-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6931509
• 关键词: Enterobacteriaceae; Nematoda; SDS polyacrylamide gel electrophoresis; bacteria infection mechanism; bacterial genetics; bacterial proteins; biological signal transduction; enteric bacteria; host organism interaction; postdoctoral investigator; protein localization; protein protein interaction; protein structure function; symbiosis; western blottings; yeast two hybrid system

DESCRIPTION (provided by applicant): The gram-negative enterobacterium Xenorhabdus nematophila mutualistically colonizes the intestines of S. carpocapsae infective juvenile nematodes. The objective of this project is a structural and functional analysis of X. nematophila Nil A, B and C membrane proteins that are required for colonization of S. carpocapsae. We hypothesize that Nil A, B and C function as a signal transduction complex, and will test this by examining interactions between the Nil proteins and other X. nematophila proteins, by determining whether the Nil proteins regulate gene expression in X. nematophila, and by deciphering the external signals recognized by the Nil proteins. Understanding molecular events underlying this model mutualistic interaction is relevant to understanding how microbes cause disease and for developing disease therapies. This statement is based on several findings: that pathogens and mutualists share common aspects in their interactions with hosts, that vertebrates and invertebrates share common aspects of immunity, which can be targeted for disease therapy, and finally, that beneficial microbes contribute to the overall health and life-styles of animals.

描述（由申请人提供）：革兰氏阴性肠杆菌嗜线虫致病杆菌在 S. carpocapsae 感染性幼年线虫的肠道中互利地定殖。该项目的目标是对 X. nematophila Nil A、B 和 C 膜蛋白进行结构和功能分析，这些膜蛋白是 S. carpocapsae 定植所需的。我们假设 Nil A、B 和 C 作为信号转导复合物发挥作用，并将通过检查 Nil 蛋白与其他嗜线虫线虫蛋白之间的相互作用、确定 Nil 蛋白是否调节嗜线虫线虫中的基因表达以及通过破译 Nil 蛋白识别的外部信号。 了解该模型互惠相互作用背后的分子事件有助于了解微生物如何引起疾病以及开发疾病疗法。该声明基于以下几个发现：病原体和共生体在与宿主的相互作用中具有共同点，脊椎动物和无脊椎动物具有共同的免疫方面，可以作为疾病治疗的目标，最后，有益微生物有助于整体健康以及动物的生活方式。