FLOW CYTOMETERS FOR ANALYSIS (FACSCALIBUR) AND SORTING: CANCER

用于分析 (FACSCALIBUR) 和分选的流式细胞仪：癌症

• 批准号: 7335012
• 负责人: David A. Thorley-Lawson
• 金额: $ 4.92万
• 依托单位: TUFTS UNIVERSITY BOSTON
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-03-01 至 2007-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7335012
• 关键词: community; flow cytometry; lasers; measurement; neoplasm /cancer

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): We are applying for funds to support the purchase of instrumentation, necessary to serve the flow cytometry needs of the research community at Tufts University School of Medicine (TUSM) and Tufts-New England Medical Center (T-NEMC). The facility has been in existence for 17 years and has been run for the last 6 years by Mr. Allen Parmelee, a skilled and respected operator, under the direct supervision of the P.I. and the Core Advisory Committee. Under their direction the facility has grown, become highly successful and maintained financial stability through competitive pricing and Departmental support. 85% of facility time is dedicated to the NIH funded work of the Core User Group. We are now asking for an LSR II (BD Biosciences) analytical machine. This instrument will double our analytical capacity, while providing access to a powerful range of techniques not currently available to us, including the measurement of intracellular calcium in cells expressing GFP variants, using Indo-1 (355nm laser); the tracking of cell cycle, viability, and proliferation, using Hoechst or DAPI (355nm laser); the analysis of protein expression, using CFP (405nm laser); the measurement of molecular association at the nanometer scale, using CFP-to-YFP FRET (405nm laser) and any analysis requiring 5 or more colors. The cost efficiency and high standards of our facility have encouraged use to the point where the facility (one FACSCalibur and one, soon to be two, MoFLos) is now over-extended. In addition, new faculty hiring, combined with growing interest in flow applications throughout TUSM and, recently, T-NEMC has increased the size of our NIH funded Core User Group from 6 to 17. This renders the acquisition of a new LSR II essential, if we are to meet the flow cytometry needs, both in time and available technology, for the community of NIH funded researchers at TUSM and T-NEMC.

该副本是利用NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构是赠款，这不一定是调查人员的机构。描述（由申请人提供）：我们正在申请资金来支持购买仪器，这是满足塔夫茨大学医学院（TUSM）和Tufts-New英格兰医学中心（T-NEMC）研究社区流式细胞仪需求的必要条件。该设施已经存在了17年，在P.I的直接监督下，熟练和受人尊敬的运营商艾伦·帕尔梅利（Allen Parmelee）先生经营了6年。和核心咨询委员会。在他们的指导下，该设施已经发展起来，通过竞争性定价和部门支持，变得非常成功并保持财务稳定。 85％的设施时间专门用于NIH资助的核心用户组的工作。我们现在要求使用LSR II（BD生物科学）分析机。该仪器将使我们的分析能力增加一倍，同时使用Indo-1（355nm Laser）提供了目前无法使用的强大技术范围，包括测量表达GFP变体细胞内的细胞内钙；使用HOECHST或DAPI（355NM激光）跟踪细胞周期，生存力和增殖；使用CFP（405nm激光）对蛋白表达的分析；使用CFP-TO-YFP FRET（405NM激光）和任何需要5种或更多颜色的分析，在纳米尺度上测量分子关联。我们设施的成本效率和高标准鼓励使用，以至于现在已经过度扩张了设施（一个Facscalibur和一个很快将是两个，Moflos）。此外，新的教师招聘，加上对整个TUSM的流动应用的日益兴趣，最近，T-NEMC将我们的NIH资助的核心用户组的规模从6增加到17。如果我们要在时间和可用的技术中满足NIH IH IH Funded Cherecters nih Funded Cherecter的NIH Chandc和THEM的TOUSM和THEM CARMC，这将使我们获得新的LSR II必不可少的必不可少的新LSR II必不可少的。