BD FACSCANTO FLOW CYTOMETER: BIOCHEMISTRY

BD FACSCANTO 流式细胞仪：生物化学

• 批准号: 7335097
• 负责人: GIRIJA MURALIDHAR
• 金额: $ 3.65万
• 依托单位: LA JOLLA INST FOR MOLECULAR MEDICINE
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7335097
• 关键词: angiogenesis; asthma; biochemistry; cell biology; chemokine; communicable diseases; computers; flow cytometry; hypersensitivity; income; intravital microscopy; medicine; neoplasm /cancer therapy; stem cells

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): The La Jolla Institute for Molecular Medicine (LJIMM) is a nonprofit research institute conducting basic and applied research in the fields of stem cell biology, asthma/allergy, chemokine biology, vaccines and therapeutics for infectious diseases, immuno therapies for cancer and asthma, signal transduction, vascular biology and angiogenesis. The flow cytometer is one of the common pieces of equipment that all the investigators use for their research projects at LJIMM. The institute currently has a FACScan with capabilities limited to detecting a maximum of three colors and is inadequate for the present needs of the investigators. Several investigators utilize off-campus multi- color flow cytometers for their work. However, it is not always practical to use equipment at off-campus locations due to high costs, time sensitive nature of the experiments, and the difficulty in transportation of samples between the institutes. Therefore, a core group of primary users of FACScan determined the specific needs of the faculty and have chosen an appropriate flow cytometer, namely FACSCanto, from BD Biosciences as the ideal system for present and future requirements. In this grant, we are requesting funds to purchase a FACSCanto system with FACSDiva program and two work stations at a total cost of $192,172. With the purchase of a FACSCanto, we will establish a Flow Cytometry Core facility, an Internal Advisory Committee (IAC) for the core and set policies for the use of the equipment. LJIMM is committed to developing core facilities and providing the necessary financial support (annual service contract and salary for a part-time research associate) and infrastructural (Server, LAN and computers) requirements. In combination with our existing Microscopy and Intravital Imaging Core, the proposed flow cytometer will be an important addition to maximize the impact of our existing research programs.

该子项目是利用 NIH/NCRR 资助的共享仪器补助金提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中出现。列出的机构是资助机构，不一定是研究者的机构。描述（由申请人提供）：拉霍亚分子医学研究所 (LJIMM) 是一家非营利性研究机构，在干细胞生物学、哮喘/过敏、趋化因子生物学、传染病疫苗和治疗、免疫学等领域开展基础和应用研究。癌症和哮喘治疗、信号转导、血管生物学和血管生成。流式细胞仪是 LJIMM 所有研究人员在其研究项目中使用的常用设备之一。该研究所目前拥有的 FACScan 只能检测最多三种颜色，不足以满足研究人员目前的需求。一些研究人员在工作中使用校外多色流式细胞仪。然而，由于成本高、实验的时间敏感性以及在研究所之间运输样本的困难，在校外使用设备并不总是可行。因此，FACScan 主要用户的核心小组确定了教师的具体需求，并选择了 BD Biosciences 的合适的流式细胞仪，即 FACSCanto，作为满足当前和未来需求的理想系统。在这笔赠款中，我们请求资金购买带有 FACSDiva 程序的 FACSCanto 系统和两个工作站，总成本为 192,172 美元。通过购买 FACSCanto，我们将建立流式细胞术核心设施、核心的内部咨询委员会 (IAC)，并制定设备的使用政策。 LJIMM 致力于开发核心设施并提供必要的财务支持（年度服务合同和兼职研究员的工资）和基础设施（服务器、局域网和计算机）需求。与我们现有的显微镜和活体成像核心相结合，拟议的流式细胞仪将是一个重要的补充，可以最大限度地发挥我们现有研究项目的影响。