Small Laboratory Animal Imaging: High Resolution Microcomputed Tomography System

小型实验动物成像：高分辨率微型计算机断层扫描系统

• 批准号: 7047441
• 负责人: RAMIRO Eliseo TORIBIO
• 金额: $ 45.78万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7047441
• 关键词: X ray; androgens; animal tissue; biomaterials; birth; bone; bone development; bone neoplasms; breast neoplasms; cell biology; cell cycle; charge coupled device camera; complement; computers; disease /disorder model; gait; genes; growth factor; income; lead; measurement; metastasis; molecular /cellular imaging; osteoblasts; osteoclasts; prostate; skull; tissue engineering; tissues; tomography; training; university

DESCRIPTION (provided by applicant): A High Resolution Microcomputed Tomography System, MicroCAT II, manufactured CTI Molecular Imaging, Inc. (Knoxville, TN) is being requested in this Shared Instrumentation Grant. The MicroCAT Imaging System was developed by the Department of Energy's Oak Ridge National Laboratories with the objective of accelerating the screening of genetically- engineered mice. The system consists of a microfocus X-ray source with a <10¿m focal spot size and larger focal spots for high-speed studies. For X-ray detection, the MicroCAT II has a high resolution 16 megapixel CCD camera with a large field of view (FOV) for screening and small FOV for high resolution imaging. The system is lead-shielded and FDA X-ray compliant. Specifications in the requested MicroCAT II system will allow NIH-funded researchers to image small laboratory animals and tissue specimens, fitting the investigators imaging needs. The MicroCAT II system bundled with the Amira Software will allow the non-invasive capture of data, generation of morphological information, longitudinal studies using landmark-based image registration applications, and high resolution 3D reconstructions necessary for disease modeling. There are no Micro-CT imaging systems at the Ohio State University. We have alternate methods to image laboratory animals and tissue specimens, however, these modalities are less sensitive for structural analysis, are time consuming, expensive to be used on a routine basis, have different applications, and do not allow longitudinal studies. A microCT system will maximize the generation of high quality information and will make the use of available resources more efficient and productive. This system will also complement the existing small animal imaging technologies. The MicroCAT II system will allow the NIH-funded investigators participating in this initiative to complete and improve their studies on bone biology, cell cycle dysregulation and development, transcriptional regulation of osteoblast and osteoclast function, prostate/breast cancer and bone metastasis, androgens and bone mass, growth factors and bone development, endocrine neoplasia and skull development, bone tissue engineering, gene delivery, and others. The MicroCAT II system will be important for in vivo longitudinal studies, avoiding the early sacrifice of valuable research animals. It will also be useful to other researchers because it can be used to image biomaterials and soft tissue interphases. This system will increase the speed and ability to detect differences not possible before, thus increasing statistical power, making possible the evaluation of a larger number of animals, and allowing in vivo repeated measurements. The instrument will be located in a readily accessible biosecured animal room (Sisson Hall) of the College of Veterinary Medicine under the supervision of a Research Scientist Supervisor (specialized in imaging) and a Laboratory Animal Veterinarian. An Organizational and Administrative Plan for the placement, maintenance, and operation of the MicroCAT II Imaging System has been formulated. An hourly fee will provide funds for a service contract and continuing education for the supervisor. Billing will be performed by the Imaging Service of the Department of Veterinary Biosciences. The principal investigator has received trained on the use of the MicroCAT II system at Oak Ridge National Labs and at ImTek, Inc. (Knoxville, TN). Users will receive training by the system manufacturer, principal investigator, supervisor, and laboratory animal veterinarian. The MicroCAT II control computer will run in a Windows XP environment connected to a high-speed real-time reconstruction engine, and linked to the local networks and the Ohio Supercomputer Center (Columbus, OH), allowing rapid data processing and access from distant sites. Salary and benefits for the Supervisor and Space and utilities for the instrument will be provided by Department of Veterinary Biosciences and the College of Veterinary Medicine.

描述（由适用提供）：高分辨率微型层析成像系统，Microcat II，制造的CTI分子成像公司，Inc。（田纳西州诺克斯维尔，田纳西州诺克斯维尔）。 Microcat成像系统是由能源部的Oak Ridge国家实验室开发的，目的是加速筛选基因工程的小鼠。该系统由一个微型聚焦X射线源组成，其焦点斑点尺寸和较大的焦点斑点用于高速研究。对于X射线检测，Microcat II具有高分辨率16百万像素CCD摄像头，具有较大的视野（FOV）用于筛选，而小型FOV则用于高分辨率成像。该系统已屏蔽，并符合FDA X射线。所需的Microcat II系统中的规格将使NIH资助的研究人员能够对小型实验室动物和组织标本进行成像，从而适合研究人员成像需求。与Amira软件捆绑在一起的Microcat II系统将允许无创捕获数据，形态学信息的产生，使用基于标志的图像注册应用的纵向研究以及对疾病建模所需的高分辨率3D重建。 俄亥俄州立大学没有Micro-CT成像系统。我们有替代方法来对实验动物和组织样本进行成像，但是，这些方式对结构分析不太敏感，耗时，常规使用，具有不同的应用，不允许纵向研究。 Microct系统将最大程度地生成高质量的信息，并使可用资源的使用效率更高和富有成效。该系统还将完成现有的小动物成像技术。 The MicroCAT II system will allow the NIH-funded investigators participating in this initiative to complete and improve their studies on bone biology, cell cycle dysregulation and development, transcriptional regulation of osteoblast and osteoclast function, prostate/breast cancer and bone metastasis, androgens and bone mass, growth factors and bone development, endocrine neoplasia and skull development, bone tissue engineering, gene delivery, and others. Microcat II系统对于避免有价值的研究动物的早期牺牲而对体内纵向研究非常重要。它对其他研究人员也将很有用，因为它可以用于成像生物材料和软组织相互作用。该系统将提高以前无法检测差异的速度和能力，从而增加统计能力，从而可以评估更多动物，并允许体内重复测量。该仪器将位于兽医学院的一位易于访问的生物抵制动物室（Sisson Hall），在研究科学家主管的监督下（专门研究成像）和实验室动物兽医。已经制定了Microcat II成像系统的放置，维护和操作的组织和行政计划。每小时费用将为主管提供服务合同和继续教育的资金。计费将由兽医生物科学系的成像服务进行。首席调查员已在Oak Ridge National Labs和Imtek，Inc。（田纳西州诺克斯维尔）的Microcat II系统中接受培训。用户将接受系统制造商，首席研究员，主管和实验室动物兽医的培训。 Microcat II控制计算机将在连接到高速实时重建引擎的Windows XP环境中运行，并链接到本地​​网络和俄亥俄州超级计算机中心（俄亥俄州哥伦布，俄亥俄州），从而可以快速数据处理和从远处站点访问。兽医生物科学系和兽医学院将为主管，太空和公用事业的工资和福利提供。