Multiphoton Imaging

多光子成像

• 批准号: 7047423
• 负责人: SUSETTE C MUELLER
• 金额: $ 50万
• 依托单位: GEORGETOWN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-15 至 2008-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7047423
• 关键词: microscopy; neoplasm /cancer; schools; training

DESCRIPTION (provided by applicant): We request funds towards the purchase of a Zeiss510/ NLO microscope with Ti:sapphire Chameleon laser. Five principle investigators from the Medical School comprise the major user group (Faden, Mueller, Pestell, Roman, Van Keuren) and 8 minor users are described including faculty from the Medical Center and the Undergraduate Campus. A total of 11 NIH-funded investigators will be supported by this application. Projects conducted by major users include: vital imaging of invasion in tissues by tumor or transgenic mammary epithelial cells; vital imaging in situ of mitochondrial function in liver; imaging temperature sensitive probes in skin; and imaging vasculogenesis in zebrafish. Minor projects include imaging the effects of stable chemotactic gradients on neuronal growth cones embedded in thick collagen gels; vital imaging of neurons and parasite infection in the gut; trafficking of G protein-coupled receptors in hypertension; live imaging of vacuolar pH within living malarial parasites; and supporting the research and educational mission of the Lombardi Comprehensive Cancer Center (LCCC) Microscopy and Imaging Shared Resource (MISR). The new microscope will be housed in the LCCC MISR. The Principle Investigator, Dr. Mueller, is an NIH funded tumor cell biologist and has been director of this facility since 1991. LCCC and Georgetown University have committed space, additional funding to meet the total purchase price and yearly service contract dollars to support the acquisition and maintenance of this instrument. Members of the major user group with microscopy expertise including the PI (Mueller) and a physicist with multiphoton expertise (Van Keuren) will be trained initially together with one of our full time MISR microscopists (Palchik). Mr. Palchik who has expertise in confocal and digital light microscopy will support use and training under the direction of Drs. Mueller and Van Keuren. The shared resource will perform associated administrative tasks including billing. Oversight will be provided by our 8 member advisory committee. In 2004, the Shared Resource was utilized by 60 investigators representing 15 departments in the Medical School and the Undergraduate Campus. MISR has an outstanding record in training. Each year since 1999, we have trained between 30- 40 LCC investigators to use the existing microscopes in the MISR. The Shared Resource is maintained by a combination of Cancer Center and grant support. Users contribute to the resource in the form of user charge back fees. There is no multiphoton microscope at Georgetown and animal imaging activities are severely curtailed as a result.

描述（由申请人提供）： 我们要求使用Ti：Sapphire Chameleon Laser购买Zeiss510/ NLO显微镜。医学院的五名主要调查人员包括主要用户组（Faden，Mueller，Pestell，Roman，Van Keuren）和8个未成年人用户，包括医疗中心和本科校园的教职员工。该申请将支持11名NIH资助的调查人员。主要用户进行的项目包括：通过肿瘤或转基因乳腺上皮细胞对组织入侵的重要成像；肝脏中线粒体功能原位的重要成像；成像皮肤中的温度敏感探针；和斑马鱼中的血管生成。次要项目包括成像稳定的趋化梯度对嵌入厚胶原蛋白凝胶中的神经元生长锥的影响；肠道中神经元和寄生虫感染的重要成像；高血压中G蛋白偶联受体的运输；活疟原虫中液泡pH的现场成像；并支持伦巴第综合癌症中心（LCCC）显微镜和成像共享资源（MISR）的研究和教育任务。新显微镜将安置在LCCC MISR中。主要调查员穆勒（Mueller）博士是NIH资助的肿瘤细胞生物学家，自1991年以来一直担任该设施的主任。LCCC和乔治敦大学（LCCC）和乔治敦大学（Georgetown University）已授予空间，以满足总购买价格和年度服务合同的额外资金和维护该乐器。具有显微镜专业知识的主要用户组成员，包括PI（MUELLER）和具有Multiphoton专业知识（Van Keuren）的物理学家（Van Keuren）的成员，最初将与我们的一位全职MISR显微镜（Palchik）一起接受培训。在共焦和数字光学显微镜方面具有专业知识的Palchik先生将在DRS的指导下支持和培训。 Mueller和Van Keuren。共享资源将执行关联的管理任务，包括计费。监督将由我们的8会员咨询委员会提供。 2004年，共享资源被60名代表医学院和本科校园的15个部门的调查员利用。 MISR在培训方面有出色的记录。自1999年以来，每年，我们都对30-40 LCC研究人员进行了培训，以使用MISR中的现有显微镜。共享资源通过癌症中心和赠款支持的结合来维持。用户以用户充电费用的形式为资源做出了贡献。乔治敦没有多光子显微镜，因此动物成像活动严重削弱了。