Cell Culture

细胞培养

基本信息

批准号：
7226095
负责人：
Gregory M Vercellotti
金额：
$ 12.92万
依托单位：
UNIVERSITY OF MINNESOTA
依托单位国家：
美国
项目类别：
财政年份：
2006
资助国家：
美国
起止时间：
2006-12-01 至 2011-11-30
项目状态：
已结题

项目摘要

The function of the cell culture core will be to provide strictly defined cells in culture for the participating investigators. The cell culture core will be responsible for the the isolation of primary cultures of human dermal microvascular endothelial cells, murine blood outgrowth endothelial cells (BOEC), murine smooth muscle cells, murine pulmonary vein and artery endothelial cells and murine fibroblasts. Cell lines including murine liver cells and human pulmonary vein and artery endothelial cells, will also be cultured. Primary cells will be grown in endotoxin-free conditions utilizing experienced cell culture techniques. The.purity of the cells will be verified using a variety of immunological techniques and functional assays. The cell culture core will distribute plates of cells to all investigators for the studies outlined in their proposals.The laboratory has rigorous techniques to provide endotoxin-free cell cultures to minimize cell activation, a critical criteria for many studies. This is accomplished by using endotoxin-free media and regular testing of all reagents used for cell culture for endotoxin using the limulus amoebocyte lysate assay. The P.I. and technician have many years of experience growing endothelial and other cells in culture. Sickle cell anemia is a devastating disease manifest by anemia, painful crises and organ injury such as stroke. The cells cultured in this Core will be used by investigators to test new treatments. By using cells, safety and effectiveness can be studied before giving new therapies to humans.

细胞培养核心的功能将是为参与的培养物提供严格定义的细胞调查人员。细胞培养的核心将负责人类原代培养物的隔离皮肤微血管内皮细胞，鼠血液生长内皮细胞（BOEC），鼠平滑肌肉细胞，鼠肺静脉和动脉内皮细胞和鼠成纤维细胞。细胞系在内鼠肝细胞以及人类肺静脉和动脉内皮细胞也将被培养。原代细胞利用经验丰富的细胞培养技术，将在无内毒素的条件下生长。细胞的植物将使用各种免疫技术和功能测定法进行验证。细胞培养核心将将细胞的板分配给所有研究人员，以进行提案中概述的研究。实验室已有严格的技术提供无内毒素细胞培养物以最大程度地减少细胞活化，这是一个关键标准许多研究。这是通过使用无内毒素培养基和对所有试剂的定期测试来完成的用于使用Limulus Amoebocyte裂解物测定法进行内毒素的细胞培养。 P.I.和技术人员有很多多年在培养中生长内皮和其他细胞的经验。镰状细胞贫血是贫血，痛苦危机和器官损伤的毁灭性疾病中风。研究人员将使用该核心中培养的细胞测试新治疗。通过使用细胞，在对人类进行新疗法之前，可以研究安全性和有效性。