Swellable Templated Copolymers

可溶胀模板共聚物

基本信息

批准号：
7075605
负责人：
Rudolf W. Seitz
金额：
$ 21.48万
依托单位：
UNIVERSITY OF NEW HAMPSHIRE
依托单位国家：
美国
项目类别：
财政年份：
2006
资助国家：
美国
起止时间：
2006-04-01 至 2010-03-31
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): We have prepared a swellable templated copolymer that selectively recognizes theophylline in the presence of caffeine, a compound that differs from theophylline by a single methyl group. The copolymer includes 10 to 20 mole-% recognition monomer and 5 mole-% crosslinker. The rest is N-isopropylacrylamide or n-propylacrylamide. This polymer undergoes a thermal phase transition, coming out of solution above the thermal phase transition temperature. Molecular recognition occurs above the thermal phase transition. We have demonstrated response to theophylline below 0.1 micromolar at room temperature and neutral pH. These copolymers are stable and easily prepared in large quantities. They offer new possibilites for designing sensors and actuators for biomedical applications. They can be used for continuous sensing of drugs or endogenous compounds. They can be employed as actuators in drug delivery systems. They can potentially be used for chemically triggered delivery of trapped compounds. This application has two specific aims. The first specific aim is to synthesize a copolymer that selectively recognizes glutamine and to incorporate this material into a sensor that is capable of in situ monitoring of glutamine in fermentation broths. Two sensing schemes are proposed. One involves measuring changes in the turbidity of membranes consisting of copolymer microspheres embedded in a hydrogel. Turbidity changes because swelling and shrinking changes copolymer refractive index. The turbidity can be measured remotely through optical fibers using components developed for optical telecommunications. The second involves incorporating donor and acceptor fluorophores into the copolymer at levels such that swelling and shrinking change the extent of fluorescence resonance energy transfer, leading to a change in the ratio of donor to acceptor fluorescence. The second is to demonstrate the feasibility of chemically triggered delivery of trapped target molecules under physiological conditions. The trigger molecule will be theophylline. The rate of release of fluorescent target molecules will be monitored as a function of time for different release conditions.

描述（由申请人提供）：我们制备了可膨胀的模板共聚物，该分子在存在咖啡因的情况下有选择地识别茶碱，该化合物与单个甲基不同的化合物与茶碱不同。共聚物包括10至20摩尔％识别单体和5摩尔％交联。其余的是N-异丙丙烯酰胺或N-丙酰丙烯酰胺。该聚合物经历了热相变，从高于热相变温度的溶液中。分子识别发生在热相跃迁之上。我们在室温和中性pH值下表现出对茶碱低于0.1微摩尔的反应。这些共聚物是稳定的，很容易大量制备。他们为设计传感器和执行器的生物医学应用提供了新的可能性。它们可用于连续传感药物或内源性化合物。它们可以在药物输送系统中用作执行器。它们有可能用于化学触发的被困化合物的化合物。该应用程序具有两个具体的目标。第一个具体目的是合成一个有选择地识别谷氨酰胺并将该材料掺入能够原位监测发酵肉汤中的谷氨酰胺的传感器中的共聚物。提出了两个传感方案。其中一种涉及测量由嵌入水凝胶中的共聚物微球组成的膜的浊度变化。浊度发生变化，因为肿胀和收缩会改变共聚物折射率。可以使用用于光学电信的组件通过光纤来远程测量浊度。第二个涉及将供体和受体荧光团纳入共聚物的水平，以使肿胀和收缩改变荧光共振能量转移的程度，从而导致供体与受体荧光之比的变化。第二个是证明在生理条件下，化学触发捕获靶标分子的可行性。触发分子将是茶碱。在不同释放条件下，将监测荧光目标分子的释放速率。