Synaptic Organization of the Basolateral Amygdala

基底外侧杏仁核的突触组织

基本信息

批准号：
7248677
负责人：
ALEXANDER JOSEPH MCDONALD
金额：
$ 30.08万
依托单位：
UNIVERSITY OF SOUTH CAROLINA AT COLUMBIA
依托单位国家：
美国
项目类别：
财政年份：
1999
资助国家：
美国
起止时间：
1999-07-01 至 2008-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7248677
关键词：
Amygdaloid structure Anatomy Axon Binding Brain Brain Stem Brain region Calcium-Binding Proteins Cell Nucleus Characteristics Cholinergic Agents Cholinergic Receptors Development Electrons Emotional Epilepsy Exhibits Fire - disasters Fluorescent Antibody Technique Functional disorder Generations Hippocampus (Brain)Interneurons Investigation Ion Channel Knowledge Laboratories Light Localized Mental disorders Methods Microscopic Muscarinics Neocortex Neurobiology Neuromodulator Neurons Neuropeptides Numbers Output Pattern Peptides Pharmaceutical Preparations Pharmacological Treatment Pharmacology Physiological Physiology Population Primates Process Property Regulation Research Research Personnel Rodent Role Serotonin Serotonin Agents Slice Stimulus Synapses System Techniques Testing Therapeutic abl Oncogene basal forebrain base cell type cholinergic nerve supply neurochemistry noradrenergic novel programs receptor research study serotonin receptor

项目摘要

DESCRIPTION (provided by applicant): The proposed study is the continuation of a long-term investigation of the neuronal and synaptic organization of the basolateral amygdala (ABL). It will focus on the regulation of ABL interneurons by transmitter-specific neuromodulator systems. Aim #1 will identify subpopulations of interneurons in the primate ABL on the basis of their content of peptides and calcium-binding proteins. These markers will be used to identify each subpopulation in Aims #2 and 3. Aim #2 will use integrated anatomical/physiological experiments to determine if different interneuronal subpopulations exhibit distinct electrophysiological characteristics. In addition, ion channels responsible for some of these electrophysiological features will be localized using immunohistochemical techniques. Aim #3 will analyze the innervation of ABL neuronal subpopulations by dopaminergic, noradrenergic, and serotonergic afferents. It will also be determined using integrated anatomical-physiological/pharmacological methods if particular interneuronal subpopulations exhibit specific serotonin receptors. Aim #4 will analyze the innervation of ABL intemeuronal subpopulations by cholinergic axons and determine which interneurons express nicotinic versus muscarinic cholinergic receptors. Additional anatomical studies in rodents will test the hypothesis that the ABL, like the neocortex and hippocampus, contains subsets of GABAergic neurons that receive inputs from, and provide outputs to, the GABAergic projection neurons of the basal forebrain. These proposed studies include the first comprehensive analysis of the electrophysiological properties of identified ABL interneuronal subpopulations in both rodent and primate brain slices. In addition, the modulation of key electrophysiological properties in these neurons by serotonin and serotonergic drugs will be investigated. These studies will contribute to an understanding of inhibitory mechanisms in the ABL and should be critical for the development of novel pharmacological treatments to modulate the activity of the ABL in epilepsy and psychiatric disorders.

描述（由申请人提供）：拟议的研究是对基底外侧杏仁核（ABL）神经元和突触组织的长期研究的延续。它将专注于通过发射机特异性神经调节器系统调节ABL中间神经元。 AIM＃1将根据其肽和钙结合蛋白的含量来确定灵长类动物ABL中神经元的亚群。这些标记将用于识别目标＃2和3中的每个亚群。目标＃2将使用集成的解剖/生理实验来确定不同的神经元间亚群是否表现出不同的电生理特征。此外，负责这些电生理特征的离子通道将使用免疫组织化学技术定位。 AIM＃3将通过多巴胺能，甲肾上腺素能和5-羟色胺能传入分析ABL神经元亚群的神经。如果特定的神经元亚群显示特定的5-羟色胺受体，则还将使用综合解剖学/药理方法确定。 AIM＃4将通过胆碱能轴突分析ABL全元素亚群的神经支配，并确定哪些中间神经元与毒蕈碱胆碱能受体相对于毒蕈碱胆碱能受体。在啮齿动物中的其他解剖学研究将检验以下假设：ABL（如新皮层和海马）中包含GABA能神经元的子集，这些神经元可以从基础前脑的GABA能投射神经元中接收输出，并提供输出。这些拟议的研究包括对啮齿动物和灵长类动物脑切片中鉴定出的ABL中神经元亚群的电生理特性的首次全面分析。此外，将研究5-羟色胺和5-羟色胺药物对这些神经元关键电生理特性的调节。这些研究将有助于理解ABL中的抑制性机制，对于开发新型药理治疗以调节ABL在癫痫和精神病中的活性至关重要。