Regulation of rRNA synthesis in corneal epithelium

角膜上皮 rRNA 合成的调控

基本信息

批准号：
6931084
负责人：
HUNG TSENG
金额：
$ 31.7万
依托单位：
UNIVERSITY OF PENNSYLVANIA
依托单位国家：
美国
项目类别：
财政年份：
2001
资助国家：
美国
起止时间：
2001-09-01 至 2006-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6931084
关键词：
DNA footprinting RNA biosynthesis cell differentiation cell type corneal epithelium genetic regulation genetic transcription in situ hybridization keratinocyte laboratory mouse nuclear runoff assay organ culture protein structure function ribosomal RNA transcription factor

项目摘要

DESCRIPTION (provided by applicant):Our long-term goal is to understand the role of basonuclin in rRNA transcription in corneal keratinocytes. This knowledge will help the elucidation of the mechanisms that regulate the proliferation of cornea! epithelium. Several transcription factors have been shown to be involved in the regulation of rRNA transcription. These factors, however, are ubiquitous and cell-type specific regulators of rRNA synthesis are still poorly understood. Basonuclin, a zinc finger protein found in abundance in the keratinocytes of stratified epithelia and the germ cells of testis and ovary, is likely to be a cell type-specific, and proliferation-related transcriptional regulator for the rRNA genes, the first of its kind. Basonuclin was detected in the neonatal and adult corneal epithelium. Our hypothesis is that basonuclin is required for the normal development and maintenance of the mouse corneal epithelium. Basonuclin enhances rRNA transcription to fulfill the demand of protein synthesis in corneal keratinocytes during development and wound healing. The following three specific aims are proposed to test this hypothesis: Specific Aim 1, To investigate if basonuclin +/- ES cells can form corneal keratinocytes in ROSA mouse chimeras. Specific Aim 2, To examine the correlation between the cellular rRNA content and the presence of basonuclin in the cornea and other ocular tissues during eye development. Specific Aim 3, To interfere with the rRNA transcription in permeabilized corneal keratinocytes by dominant-negative reagents of basonuclin function.

描述（由申请人提供）：我们的长期目标是了解 Basonuclin在角膜角质形成细胞中rRNA转录中的作用。这知识将有助于阐明调节的机制角膜的扩散！上皮。几个转录因素已经显示与rRNA转录的调节有关。这些因素，但是，无处不在和细胞类型的rRNA合成调节剂是仍然很了解。 Basonuclin，一种丰富的锌指蛋白在分层上皮和睾丸生殖细胞的角质形成细胞中卵巢，可能是特定于细胞类型的，并且与增殖有关 rRNA基因的转录调节剂，第一个。 basonuclin 在新生儿和成年角膜上皮中检测到。我们的假设是，正常发育和维持小鼠角膜上皮。 Basonuclin增强了rRNA 转录以满足角膜中蛋白质合成的需求角质形成细胞在发育和伤口愈合过程中。以下三个提出了具体目的来检验这一假设：特定的目标1，以研究Basonuclin +/- ES细胞是否形成角膜罗莎小鼠嵌合体中的角质形成细胞。特定目标2，以检查细胞rRNA含量之间的相关性以及在角膜和其他眼组织中存在盆地的存在眼睛发育。特定目标3，干扰透明的rRNA转录 Basonuclin功能的主要阴性试剂的角膜角质形成细胞。