PixCell IIe Laser Capture Microdissection Instrument

PixCell IIe 激光捕获显微切割仪

• 批准号: 6877417
• 负责人: CAROL L ROSENBERG
• 金额: $ 13.32万
• 依托单位: BOSTON MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6877417
• 关键词: biomedical equipment purchase; cell type; laser capture microdissection; molecular /cellular imaging; tissues

DESCRIPTION (provided by applicant): This application requests funds for state-of-the-art laser capture microdissection (LCM) equipment, specifically, a Pixcell lle LCM instrument (Arcturus Engineering, Mountain View, CA). Laser capture microdissection is a powerful new technology for study of in vivo tissues. It permits isolation of particular cell types, or cells from particular locations, from complex or heterogeneous tissues. Subsequently, nucleic acids or proteins can be extracted from the microdissected cells, and analyzed. This institution's only LCM apparatus is the prototypic and outdated Pixcell I, purchased in 1998. It has been heavily and productively used, resulting in publications, funding, and promising preliminary data. However, the instrument is obsolete: the beam-width is wide, the software does not interface with current programs and as of 9/03 the company no longer provides maintenance or support for this model. Demand is strong for an up-to-date instrument with better optics and software, which is capable of dissecting smaller areas and fluorescent-labeled cells. Therefore, the Pixcell's current major users, along with additional investigators, request support for an updated LCM instrument. The major users are productive, NIH-funded investigators, experienced with prototype equipment, for whose biomedical research the ability to isolate small homogeneous cell populations is fundamental. The institution, recognizing the need for this instrument has committed support to maintain this critical research resource. This constellation seems ideally suited to the purposes of the NCRR SIG program.

描述（由申请人提供）：此申请要求用于最先进的激光捕获微解剖（LCM）设备的资金，特别是PixCell LLE LCM仪器（Arcturus Engineering，Mountain Vieens，CA）。 激光捕获显微解剖是一种用于研究体内组织的强大新技术。它允许从复杂或异质组织中分离特定的细胞类型或特定位置的细胞。随后，可以从微分解析的细胞中提取核酸或蛋白质，并进行分析。该机构唯一的LCM设备是1998年购买的原型和过时的PixcellI。它已被大量和有效地使用，从而获得了出版物，资金和有希望的初步数据。但是，该仪器已经过时了：梁宽度很宽，软件不与当前程序接口，并且从9/03开始，该公司不再为此模型提供维护或支持。对于具有更好的光学和软件的最新仪器而言，需求是强大的，该仪器能够剖析较小的区域和荧光标记的细胞。 因此，PixCell目前的主要用户以及其他调查人员要求支持更新的LCM仪器。主要用户是富有成效的NIH资助的研究人员，具有原型设备的经验，其生物医学研究能够隔离小均质细胞群体的能力。该机构认识到对该工具的需求已致力于维持这一关键研究资源。该星座似乎理想地适合NCRR SIG计划的目的。

项目成果

Gene expression profiles of estrogen receptor-positive and estrogen receptor-negative breast cancers are detectable in histologically normal breast epithelium.

• DOI: 10.1158/1078-0432.ccr-10-1369
• 发表时间: 2011-01-15
• 期刊: Clinical cancer research : an official journal of the American Association for Cancer Research
• 作者: Graham K;Ge X;de Las Morenas A;Tripathi A;Rosenberg CL
• 通讯作者: Rosenberg CL