Transgenic & Bio-Behavioral Measurement Animal Facility

转基因

• 批准号: 6905198
• 负责人: ELIAS K MICHAELIS
• 金额: $ 63.5万
• 依托单位: UNIVERSITY OF KANSAS LAWRENCE
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-08-15 至 2007-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6905198
• 关键词: animal colony; building /facility design /renovation; genetically modified animals; laboratory mouse

DESCRIPTION (provided by applicant): Many of the faculty at the University of Kansas have maintained an excellent tradition of conducting basic biomedical research on the main campus in Lawrence and, through collaborations with clinical scientists at the Medical Center campus, have been involved in transitional research in areas such as mental retardation, aging, Alzheimer's Disease, neurological diseases, pregnancy and early development, and cancer. The University plans to construct two major, bi-campus, multidisciplinary research institutes, one in neuroscience and the other in cancer research. These institutes will combine the efforts of 50 neuroscientists and 30 cancer researchers. Both institutes have identified the development of experimental animal models for neuro-development, neurological diseases, and cancer biology as a key component in achieving an understanding of basic biological mechanisms for neurological diseases and cancer and for developing new therapeutic interventions. Transgenic and gene knockout approaches in mice are the main pathway to such animal model development. The focus of this proposal is on renovating space within the main animal care facility at the University of Kansas in Lawrence to be used to generate, house, and breed transgenic and gene knockout mice. Over the past two years a microinjection laboratory was established and personnel trained to generate transgenic mice. Limited rodent housing was designated for mouse housing and breeding rooms for transgenic animals and a small bio-behavioral laboratory configured to allow for sensitive measures of changes in behavioral and neurological function as a result of over-expression or disruption of various genes. However, these facilities are not optimal for the conduct of the experimental work planned or for the housing of major instrumentation that will be purchased in fiscal year 2005. They also are not centralized in a manner that would effectively support existing and future PHS-funded research. This proposal has as its main focus the renovation/restructuring of a space of approximately 5,600 gross square feet so that it will become a centralized transgenic/gene knockout facility that will serve the community of scientists of the whole University. A unique feature of this facility besides the laboratory for micro-injection, mouse housing/breeding and mouse genotyping, is the inclusion of a well designed space for bio-behavioral measurements and one for complete pathological examination of new transgenic/knockout mice generated.

描述（由申请人提供）： 堪萨斯大学的许多教师保持了在劳伦斯主校区进行基础生物医学研究的优良传统，并通过与医学中心校区的临床科学家合作，参与了精神发育迟滞等领域的过渡性研究、衰老、阿尔茨海默病、神经系统疾病、怀孕和早期发育以及癌症。该大学计划建设两个主要的、双校区的、多学科的研究机构，一个是神经科学研究机构，另一个是癌症研究机构。这些研究所将汇集 50 名神经科学家和 30 名癌症研究人员的努力。两个研究所均已确定神经发育、神经系统疾病和癌症生物学实验动物模型的开发是了解神经系统疾病和癌症的基本生物学机制以及开发新的治疗干预措施的关键组成部分。小鼠转基因和基因敲除方法是此类动物模型开发的主要途径。该提案的重点是翻新劳伦斯堪萨斯大学主要动物护理设施内的空间，用于生成、饲养和繁殖转基因和基因敲除小鼠。在过去的两年里，显微注射实验室成立了，并对人员进行了培育转基因小鼠的培训。有限的啮齿类动物饲养室被指定为转基因动物的小鼠饲养室和繁殖室，以及一个小型生物行为实验室，该实验室被配置为可以敏感地测量由于各种基因的过度表达或破坏而导致的行为和神经功能的变化。然而，这些设施对于进行计划的实验工作或容纳 2005 财年购买的主要仪器来说并不是最佳选择。它们也没有以有效支持现有和未来 PHS 资助的研究的方式集中化。 。该提案的主要重点是对约 5,600 平方英尺的空间进行改造/重组，使其成为一个集中的转基因/基因敲除设施，为整个大学的科学家群体服务。除了用于显微注射、小鼠饲养/饲养和小鼠基因分型的实验室外，该设施的一个独特之处是包括一个精心设计的生物行为测量空间和一个用于对新生成的转基因/基因敲除小鼠进行完整病理检查的空间。