Symbiotic colonization of host mucus by Vibrio fischeri

费氏弧菌在宿主粘液中的共生定殖

• 批准号: 7258844
• 负责人: Mark J Mandel
• 金额: $ 4.88万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至 2009-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7258844
• 关键词: Address; Animals; Bacteria; Benign; Environment; Generations; Goals; Hawaiian population; Image; Infection; Laser Scanning Confocal Microscopy; Life; Mediating; Molecular; Molecular Genetics; Morphology; Mucous body substance; Play; Process; Research; Role; Role playing therapy; Site; Species Specificity; Squid; Surface; Symbiosis; Vibrio; Vibrio fischeri; hatching; insight; research study

DESCRIPTION (provided by applicant): The objective of this research is to elucidate the mechanisms by which animals acquire bacterial symbionts anew during each generation. To reach this goal I will take advantage of the natural association between the Hawaiian bobtail squid, Euprymna scolopes, and its specific, luminescent symbiont, Vibrio fischeri. The morphology of the symbiosis facilitates direct access to the site of infection, allowing for imaging of live bacteria as they colonize nascent squid. The experiments proposed combine molecular genetic approaches with confocal laser-scanning microscopy to address the following specific aims: (1) identification of factors in V. fischeri that mediate the initiation of the symbiosis, and (2) examination of the role played by host-derived mucus in establishing the symbiosis and determining bacterial species-specificity. Colonization of mucosal surfaces by pathogenic Vibrio species is a critical process for infection. This benign infection provides a natural environment in which to study these processes with minimal perturbation, which may also yield insights into how pathogenic infections are mediated in a variety of hosts.

描述（由申请人提供）：本研究的目的是阐明动物在每一代中重新获得细菌共生体的机制。为了实现这一目标，我将利用夏威夷短尾乌贼、Euprymna scolopes 与其特定的发光共生体费氏弧菌之间的自然联系。共生的形态有利于直接进入感染部位，从而可以在活细菌定殖于新生鱿鱼时对其进行成像。实验建议将分子遗传学方法与共聚焦激光扫描显微镜相结合，以实现以下具体目标：（1）鉴定费氏弧菌中介导共生启动的因子，以及（2）检查宿主所起的作用衍生的粘液建立共生和确定细菌物种特异性。致病性弧菌在粘膜表面的定植是感染的关键过程。这种良性感染提供了一个自然环境，可以在其中以最小的扰动研究这些过程，这也可能有助于了解致病性感染如何在各种宿主中介导。