Trypanosome RNA Binding Proteins

锥虫 RNA 结合蛋白

• 批准号: 7217432
• 负责人: Noreen Williams
• 金额: $ 36.8万
• 依托单位: STATE UNIVERSITY OF NEW YORK AT BUFFALO
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-15 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7217432
• 关键词: Address; Affect; Binding; Biochemical; Blood Circulation; Cell Line; Cell Nucleolus; Cell Nucleus; Cell physiology; Cells; Chemicals; Development; Elements; Epitopes; Family; Goals; Homologous Protein; Immunoprecipitation; Intervention; Knock-out; Lead; Life Cycle Stages; Light; Modeling; Molecular; Mutation; Nature; Nucleolar Proteins; Organism; Play; Post-Translational Regulation; Process; Protein Binding; Proteins; RNA Interference; RNA Processing; RNA-Binding Proteins; Regulation; Research; Research Personnel; Ribosomal RNA; Role; Staging; Transcript; Translational Regulation; Trypanosoma; Trypanosoma brucei brucei; Untranslated Regions; Work; Yeasts; knock-down; mRNA Stability; nucleic acid binding protein; programs; protein expression; protein function; transmission process; yeast two hybrid system

DESCRIPTION (provided by applicant): The objective of this proposal is to further characterize the function and developmental regulation of two highly related nucleic acid binding proteins, p34 and p37, from the parasitic protozoan, Trypanosoma brucei. We have recently shown that these proteins are associated with 5 S rRNA. We have further shown that these proteins also interact with a family of nucleolar proteins, the NOPP44/46 proteins. These two RNA binding proteins show significant developmental regulation and although the proteins are highly homologous they are regulated by different mechanisms, p34 is predominantly expressed in procyclic forms and is regulated at the level of mRNA stability, p37 is predominantly expressed in the bloodstream stage and is regulated by translational control and protein stability. The specific aims of the project are to: 1. Determine whether the two homologous RNA binding proteins, p34 and p37, have distinct roles in the trypanosome, particularly in light of their differential regulation. 2. Determine the mechanism of posttranscriptional regulation for the expression of the p34 protein. 3. Determine the mechanisms of translational regulation of expression for the p37 protein. These experimental approaches will lead to a better understanding of the function and significance of these two unique nucleic acid binding proteins in cellular RNA processing. Given the developmental nature of their expression, we may find them to be critical to the ability of T. brucei to survive transmission from one host type to another. This may provide a strategic target for chemical intervention.

描述（由申请人提供）：该建议的目的是进一步表征来自寄生虫原生动物Brucei的两个高度相关的核酸结合蛋白p34和p37的功能和发育调节。我们最近表明，这些蛋白质与5 s rRNA有关。我们进一步表明，这些蛋白质还与核仁蛋白（NOPP44/46蛋白）的家族相互作用。这两种RNA结合蛋白显示出明显的发育调节，尽管蛋白质高度同源，它们受不同的机制调节，但p34主要以procyclic形式表达，并且在mRNA稳定性水平下受到调节，但p37主要在血液中表达，并在血液中表达，并受到翻译控制和蛋白质稳定的调节。该项目的具体目的是： 1。确定两个同源RNA结合蛋白P34和p37是否在锥虫体中具有不同的作用，尤其是鉴于它们的差分调节。 2。确定p34蛋白表达后调节后调节的机制。 3。确定p37蛋白表达转化调节的机制。 这些实验方法将更好地理解这两种独特的核酸结合蛋白在细胞RNA加工中的功能和意义。鉴于其表达的发展性质，我们可能会发现它们对于t. brucei的能力至关重要。这可以为化学干预提供一个战略目标。