Photofragmentation MS of Ion Mobility Separated Peptides

离子淌度分离肽的光裂解质谱

• 批准号: 6994041
• 负责人: Kelley L Waters
• 金额: $ 49.05万
• 依托单位: IONWERKS, INC.
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2007-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6994041
• 关键词: bioengineering /biomedical engineering; biotechnology; high throughput technology; ion transport; mass spectrometry; matrix assisted laser desorption ionization; method development; molecular /cellular imaging; peptides; physical separation; protein structure; protein transport; proteomics; small molecule; structural biology

DESCRIPTION (provided by applicant): Ion mobility (IM) is a perfect instrumental platform for slowing and separating peptide ions-this innovation has enabled efficient VUV photofragmentation (IM-oTOFMS/MS) to be demonstrated in phase I. VUV photofragmentation yields a near perfect technology for "on demand" structural fragment ion analysis of complex peptide mixtures especially when combined with the proven capability of IM to distinguish phosphorylated vs. unphosphorylated peptides. In addition, recent internally funded research at lonwerks [34-37] has rather unexpectedly proved the efficacy of incorporating nanoparticulate MALDI matrices into the near surface of biotissues. Recently, lonwerks publications [34-40] and patents [41-44] have established a new approach to molecular microprobe imaging of biotissue surfaces. Thus, the simplification of complex laser microprobe molecular ion spectra from particulate treated biosurfaces is another application which will also benefit from the combination of ion mobility separation followed with on demand photofragmentation structural analysis. Phase II will develop unique IM-oTOFMS/MS hardware using patented multi-pixel position sensitive detectors in a specially designed oTOFMS. Enhanced mass and mobility resolution as well as photofragment MS of this instrument will be tested for applications in proteomics and tissue imaging. Commercialization depends on showing that only IMS-oTOFMS/MS can perform these applications.

描述（由申请人提供）：离子淌度 (IM) 是用于减慢和分离肽离子的完美仪器平台 - 这项创新使高效的 VUV 光断裂 (IM-oTOFMS/MS) 能够在第一阶段得到验证。VUV 光断裂产生近乎复杂肽混合物“按需”结构碎片离子分析的完美技术，尤其是与 IM 经验证的区分磷酸化和非磷酸化肽的能力相结合。此外，lonwerks 最近的内部资助研究[34-37]出乎意料地证明了将纳米颗粒 MALDI 基质纳入生物组织近表面的功效。最近，lonwerks 出版物 [34-40] 和专利 [41-44] 建立了一种生物组织表面分子微探针成像的新方法。因此，来自颗粒处理的生物表面的复杂激光微探针分子离子光谱的简化是另一个应用，该应用也将受益于离子淌度分离和按需光碎裂结构分析的组合。第二阶段将开发独特的 IM-oTOFMS/MS 硬件，在专门设计的 oTOFMS 中使用获得专利的多像素位置敏感探测器。该仪器的增强质量和迁移率分辨率以及照片碎片 MS 将在蛋白质组学和组织成像中进行应用测试。商业化取决于证明只有 IMS-oTOFMS/MS 才能执行这些应用。