Photofragmentation MS of Ion Mobility Separated Peptides

离子淌度分离肽的光裂解质谱

• 批准号: 6994041
• 负责人: Kelley L Waters
• 金额: $ 49.05万
• 依托单位: IONWERKS, INC.
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2007-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6994041
• 关键词: bioengineering /biomedical engineering; biotechnology; high throughput technology; ion transport; mass spectrometry; matrix assisted laser desorption ionization; method development; molecular /cellular imaging; peptides; physical separation; protein structure; protein transport; proteomics; small molecule; structural biology

DESCRIPTION (provided by applicant): Ion mobility (IM) is a perfect instrumental platform for slowing and separating peptide ions-this innovation has enabled efficient VUV photofragmentation (IM-oTOFMS/MS) to be demonstrated in phase I. VUV photofragmentation yields a near perfect technology for "on demand" structural fragment ion analysis of complex peptide mixtures especially when combined with the proven capability of IM to distinguish phosphorylated vs. unphosphorylated peptides. In addition, recent internally funded research at lonwerks [34-37] has rather unexpectedly proved the efficacy of incorporating nanoparticulate MALDI matrices into the near surface of biotissues. Recently, lonwerks publications [34-40] and patents [41-44] have established a new approach to molecular microprobe imaging of biotissue surfaces. Thus, the simplification of complex laser microprobe molecular ion spectra from particulate treated biosurfaces is another application which will also benefit from the combination of ion mobility separation followed with on demand photofragmentation structural analysis. Phase II will develop unique IM-oTOFMS/MS hardware using patented multi-pixel position sensitive detectors in a specially designed oTOFMS. Enhanced mass and mobility resolution as well as photofragment MS of this instrument will be tested for applications in proteomics and tissue imaging. Commercialization depends on showing that only IMS-oTOFMS/MS can perform these applications.

描述（由申请人提供）：离子迁移率（IM）是放缓和分离肽离子离子的理想工具平台 - 这项创新已使有效的VUV光碎片（IM-OTOFMS/MS）在I期中证明I。VUV光照射差异差异近距离杂交的技术，尤其是在需求碎片的过程中，尤其是在结构上杂乱的混合物，尤其如此磷酸化与未磷酸化的肽。此外，最近在Lonwerks的内部资助研究[34-37]相当出乎意料地证明了将纳米构成的MALDI矩阵纳入生物疗法的近表面的功效。最近，Lonwerks Publications [34-40]和专利[41-44]建立了一种新方法来对生物学表面的分子微探针成像。因此，从颗粒处理的生物表面上简化复杂激光微探针分子离子光谱是另一种应用，它也将受益于离子迁移率分离的组合，然后按需按需光裂差结构分析。第二阶段将使用专门设计的OTOFMS中使用专利的多像素位置敏感探测器开发独特的IM-OTOFMS/MS硬件。该仪器的质量和迁移率分辨率以及该仪器的光碎片MS将进行测试，以用于蛋白质组学和组织成像中的应用。商业化取决于表明只有IMS-OTOFMS/MS才能执行这些应用程序。