Antigen Identification in Occult Chorioretinopathies

隐匿性脉络膜视网膜病变中的抗原鉴定

• 批准号: 7039013
• 负责人: Jeffrey L Bennett
• 金额: $ 15.04万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7039013
• 关键词: affinity chromatography; antibody specificity; antigen antibody reaction; antiserum; biomarker; chorioretinitis; choroid uvea; choroiditis; clinical research; confocal scanning microscopy; enzyme linked immunosorbent assay; eye disorder diagnosis; genetic library; genetic screening; human subject; in situ hybridization; molecular cloning; nucleic acid sequence; open reading frames; pathologic process; peptide library; polymerase chain reaction; retina disorder; serology /serodiagnosis; uveitis; western blottings

DESCRIPTION (provided by applicant): Acute zonal occult outer retinopathy, multiple evanescent white dot syndrome, acute macular neuroretinopathy, acute idiopathic blind spot enlargement syndrome, multifocal choroiditis, punctuate inner choroidopathy, and diffuse subretinal fibrosis syndrome are a group of chorioretinal inflammatory disorders of unknown etiology. These disorders possess common clinical features, and affected individuals may evolve from one condition to another during their clinical course. Vision loss may vary from mild to severe. To date, however, there are no absolute criteria to define disease subtypes, no available markers to diagnose disease or gauge prognosis, and no effective therapies. As a result, debate remains as to whether these disorders represent a related spectrum of inflammatory chorioretinopathies. Pathologic specimens have revealed a B-cell predominant inflammatory infiltrate with antibody deposition providing a rationale for our hypothesis that antibodies play a role in the pathogenesis of these disorders and are directed against retinal or choroidal antigens that trigger or propagate disease. To identify an antigen specific to occult chorioretinopathies (OC) we will: (1) identify clones in a human uveal cDNA expression Library and a random peptide library whose products react with serum from OC patients; (2) determine the disease-specificity of candidate QC-specific antigens and peptides by screening sera from patients with QC and control ocular uveitides; and (3) characterize, using molecular biologic techniques, OC-specific clones and study their pattern of expression in the retina and choroid. Identification of OC-specific markers will help classify these occult inflammatory disorders as a specific nosologic entity, generate biologic markers to diagnose preclinical and clinical disease, and develop therapeutic strategies to prevent loss of vision in affected individuals.

描述（由申请人提供）：急性区域倾向外部视网膜病，多个evaneScent的白点综合征，急性黄斑神经性疾病，急性特发性盲点肿块综合征，多因子脉络膜炎，多灶性脉络膜炎，注点内部的脉络膜病和差异性纤维纤维症状综合症，综合综合症。病因。这些疾病具有共同的临床特征，受影响的个体在临床过程中可能会从一种疾病发展到另一种疾病。视力丧失可能从轻度到重度不等。然而，迄今为止，还没有确定疾病亚型的绝对标准，没有可用的标记来诊断疾病或仪表预后，也没有有效的疗法。 结果，关于这些疾病是否代表相关炎性脉络膜毒素病谱的争论仍然存在。 病理标本已经显示出B细胞主要的炎症性浸润性抗体沉积为我们的假设提供了理由，即抗体在这些疾病的发病机理中起作用，并针对触发或传播疾病的视网膜或脉络膜抗原发挥作用。 为了识别特异性的神秘脉络膜毒素病（OC）的抗原（OC）：（1）识别人紫veal cDNA表达文库中的克隆和随机肽库中的产物与OC患者的血清反应的随机肽库； （2）通过筛查QC和对照眼葡萄肽患者的血清来确定候选QC特异性抗原和肽的疾病特异性； （3）使用分子生物学技术，OC特异性克隆来表征其在视网膜和脉络膜中的表达方式。 OC特异性标志物的识别将有助于将这些隐秘炎症性疾病分类为特定的病因实体，生成生物学标记以诊断临床前和临床疾病，并制定治疗策略，以防止受影响个体的视力丧失。

项目成果

Optic neuritis and the neuro-ophthalmology of multiple sclerosis.

视神经炎和多发性硬化症的神经眼科。

• DOI: 10.1016/s0074-7742(07)79028-1
• 发表时间: 2007
• 期刊: International review of neurobiology
• 作者: Kaur,Paramjit;Bennett,JeffreyL
• 通讯作者: Bennett,JeffreyL