ADP-ribosylation in plant-bacteria interactions

植物-细菌相互作用中的 ADP-核糖基化

• 批准号: 7108814
• 负责人: LORI C ADAMS-PHILLIPS
• 金额: $ 4.88万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7108814
• 关键词: ADP ribosylation; Arabidopsis; Pseudomonas; bacteria infection mechanism; bacterial genetics; cell death; enzyme activity; genetically modified plants; host organism interaction; immunity; pentosyltransferase; plant genetics; plants; postdoctoral investigator; pyrophosphatase; transcription factor

DESCRIPTION (provided by applicant): The present project will examine how effector proteins of bacterial pathogens attack plants and cause pathogenic outcomes, and how the plant counters these attacks. My recent work on Arabidopsis defense responses, findings from labs cataloguing Pseudomonas syringae effector proteins, and work on bacterial pathogens of mammals all point to the relevance of ADP-ribosylation in plant-bacteria interactions. This is a new, largely unstudied area of plant pathogenesis. Three Specific Aims are proposed: 1) The contributions of recently implicated plant ADP-ribose pyrophosphatase and plant poly(ADP-ribose) glycohydrolase to plant disease resistance will be examined. 2) Bacterial ADP-ribosylation of plant host targets will be studied. The ADP-ribosylation activity of three P. syringae ADP-ribosyltransferase effector proteins will be determined, the respective contributions of these effectors to virulence will be examined, and their plant protein targets will be identified. 3) The role of endogenous poly(ADP-ribosyl)ation in plant defense and HR cell death will be investigated. Plant diseases threaten human sustenance and serve as an informative model for host-pathogen interactions research. The proposed work opens new areas highly suitable for future independent research.

描述（由申请人提供）：本项目将研究细菌病原体的效应蛋白如何攻击植物并导致致病结果，以及植物如何抵抗这些攻击。我最近对拟南芥防御反应的研究、对丁香假单胞菌效应蛋白进行分类的实验室发现以及对哺乳动物细菌病原体的研究都表明了 ADP-核糖基化在植物-细菌相互作用中的相关性。这是植物发病机制的一个新的、很大程度上未经研究的领域。提出了三个具体目标： 1）将检查最近涉及的植物 ADP-核糖焦磷酸酶和植物聚（ADP-核糖）糖水解酶对植物抗病性的贡献。 2) 将研究植物宿主靶标的细菌ADP-核糖基化。将测定三种丁香假单胞菌 ADP-核糖基转移酶效应蛋白的 ADP-核糖基化活性，检查这些效应蛋白对毒力的各自贡献，并鉴定其植物蛋白靶点。 3)将研究内源性多(ADP-核糖基)化在植物防御和HR细胞死亡中的作用。植物病害威胁着人类的生计，并可作为宿主与病原体相互作用研究的信息模型。拟议的工作开辟了非常适合未来独立研究的新领域。