Core B--Immunological Monitoring

核心B——免疫监测

• 批准号: 7189870
• 负责人: DIANA M METES
• 金额: $ 49.42万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-02-01 至 2009-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7189870
• 关键词: Epstein Barr virus; T lymphocyte; biomedical facility; clinical research; heart transplantation; histocompatibility typing; human subject; immunologic substance resource /registry /referral center; lung transplantation; pediatrics; transplantation immunology

The Immunological Monitoring Core B is a very essential component of the SCCOR grant. This core will assist all four scientific projects and will provide the necessary information to the other cores. The Specific Aims of this core are: Aim 1: To procure, triage and store peripheral blood mononuclear cells, plasma and serum, and endomyocardial biopsy tissue specimens. Aim 2: To process the specimens as required by individual investigators for experimental protocols in the clinical and basic projects. Aim 3: To establish the human lymphoblastoid cell lines (LCL) from peripheral blood for each patient, and maintain the cell lines for EBV in vitro studies. Aim 4: To develop, evaluate and perform in vitro monitoring assays to determine the frequency and activity of immune effector cells in order to support basic and clinical projects. The core will make available to the investigators the specimens obtained from pediatric heart and heart-lung transplant recipients participating in the various studies. In order to assess the immune modulation effect of biotherapies and/or impact of elevation of EBV load, these serial specimens will be tested in a panel of assays developed and validated by the Core. These assays will identify, quantitate and characterize the allo-reactive T-cells in the circulation. Other assays will measure and characterize the immune defects of EBV-specific T cells in peripheral blood of these patients. The development of such assays and their application to assess of immune functions in the course of therapy are essential components of the Core. The Core will also perform HLA DNA typing required for projects 1-3.

免疫监测核心 B 是 SCCOR 资助的一个非常重要的组成部分。该核心将协助所有四个科学项目，并向其他核心提供必要的信息。该核心的具体目标是： 目标1：采购、分类和储存外周血单核细胞、血浆和血清，以及 心内膜心肌活检组织标本。 目标 2：按照临床和基础项目中实验方案的个别研究者的要求处理标本。 目标3：从每位患者的外周血中建立人淋巴母细胞系（LCL），并维持细胞系用于EBV体外研究。 目标 4：开发、评估和进行体外监测测定，以确定免疫效应细胞的频率和活性，以支持基础和临床项目。 该核心将为研究人员提供从参与各种研究的儿科心脏和心肺移植受者身上获得的标本。为了评估生物疗法的免疫调节作用和/或 EBV 负荷升高的影响，这些系列样本将在由 Core 开发和验证的一组测定中进行测试。这些检测将识别、定量和表征循环中的同种异体反应性 T 细胞。其他检测将测量和表征这些患者外周血中 EBV 特异性 T 细胞的免疫缺陷。此类测定的开发及其在治疗过程中评估免疫功能的应用是核心的重要组成部分。核心将 还执行项目 1-3 所需的 HLA DNA 分型。