Small Animal Imaging of Prostate Cancer Therapies

前列腺癌治疗的小动物成像

• 批准号: 7095900
• 负责人: AMY L VAVERE
• 金额: $ 4.88万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-07-01 至 2007-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7095900
• 关键词: androgen inhibitor; androgen receptor; antineoplastics; athymic mouse; autoradiography; bioimaging /biomedical imaging; blood flow measurement; cell proliferation; gene expression; glucose metabolism; hypoxia; immunocytochemistry; male; neoplasm /cancer chemotherapy; nonhuman therapy evaluation; positron emission tomography; postdoctoral investigator; prostate neoplasms; protein structure function; radionuclide imaging /scanning; radiotracer; transcription factor

DESCRIPTION (provided by applicant): This investigation would explore a hypothesis regarding the relationship in prostate cancer between androgen receptors, anti-androgen therapy, HIF-1 alpha and hypoxia in an animal model of direct relevance to humans. HIF-1 expression has already been shown to be a direct marker of oxygenation in tumors. Direct imaging of this protein would not only provide further insight into prostate cancer biochemistry, but also aid clinicians in designing appropriate treatment schemes. This proposal is aimed at delineating the relationship between androgen ablation therapy, hypoxia, and HIF-1 alpha expression while also monitoring changes in blood flow and metabolism in animal models of prostate cancer. The specific aims, in brief, are (1) To utilize microPET imaging to delineate changes in hypoxia, blood flow, metabolism, androgen receptor expression, cellular proliferation, and HIF-1 alpha expression in animal models of prostate cancer; (2) To conjugate an anti-HIF-1 alpha antibody with DOTA and label with 64Cu; and (3) To correlate immunohistochemical and autoradiographic data of HIF-1 alpha expression with imaging results.

描述（由申请人提供）： 这项研究将在与人类直接相关的动物模型中探索关于前列腺癌中雄激素受体、抗雄激素治疗、HIF-1α和缺氧之间关系的假设。 HIF-1 表达已被证明是肿瘤氧合的直接标志。 这种蛋白质的直接成像不仅可以进一步了解前列腺癌的生物化学，还可以帮助临床医生设计适当的治疗方案。 该提案旨在描绘雄激素消融治疗、缺氧和 HIF-1 α 表达之间的关系，同时监测前列腺癌动物模型中血流和代谢的变化。 简而言之，具体目标是（1）利用 microPET 成像描绘前列腺癌动物模型中缺氧、血流、代谢、雄激素受体表达、细胞增殖和 HIF-1 α 表达的变化； (2)将抗HIF-1α抗体与DOTA缀合，并用64Cu标记； (3)将HIF-1α表达的免疫组织化学和放射自显影数据与成像结果相关联。

项目成果

Examining the relationship between Cu-ATSM hypoxia selectivity and fatty acid synthase expression in human prostate cancer cell lines.

检查人类前列腺癌细胞系中 Cu-ATSM 缺氧选择性与脂肪酸合酶表达之间的关系。

• DOI: 10.1016/j.nucmedbio.2007.11.012
• 发表时间: 2008
• 期刊: Nuclear medicine and biology
• 影响因子: 3.1
• 作者: Vāvere,AmyL;Lewis,JasonS
• 通讯作者: Lewis,JasonS