CORE--ANIMAL MODELS

核心--动物模型

• 批准号: 6825451
• 负责人: YVETTE FRANCE TACHE
• 金额: $ 7.67万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-12-01 至 2009-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6825451
• 关键词: animal colony; biomedical facility; digestive disorder; disease /disorder model; enteric nervous system; experimental designs; gene mutation; laboratory mouse; laboratory rat; neural information processing

The overall objective of the Animal Models Core is to provide expertise to members of the CURE: Digestive Diseases Research Core Center (CURE: DDRCC) for in vivo characterization of normal and pathophysiological mechanisms of hormonal and neural regulation of gastrointestinal (GI) function and brain-gut interactions in rodents. The specific objectives are to provide CURE: DDRCC investigators access to: (1) in vivo experimental models to assess gastric and intestinal function in rats and mice; (2) specialized facilities and equipment to measure GI secretions, motor function, blood flow, resistance of the mucosa to injury and functional mapping of neuronal activity at the cellular level; (3) methods for in vivo administration of peptides, neurotransmitters, and drugs, sampling blood and body fluids, and tissue collection; (4) methods to assess afferent and efferent arms of the neural pathways involved in brain-gut interactions; (5) expertise in protocol design, data analysis and program development for analysis of electrophysiological traces; and (6) transgenic animals with specific gene mutations through interaction with transgenic Cores within UCLA.

动物模型核心的总体目标是为 CURE 成员提供专业知识： 消化疾病研究核心中心（CURE：DDRCC），负责啮齿类动物胃肠道（GI）功能和脑肠相互作用的激素和神经调节的正常和病理生理机制的体内表征。具体目标是为 CURE：DDRCC 研究人员提供：（1）评估大鼠和小鼠胃和肠道功能的体内实验模型； (2) 测量胃肠道分泌物、运动功能、血流量、粘膜抗损伤能力和细胞水平神经元活动功能图谱的专用设施和设备； （三）肽、神经递质和药物的体内给药、血液和体液采样以及组织采集的方法； (4) 评估脑肠神经通路传入和传出臂的方法 互动； (5) 电生理痕迹分析的方案设计、数据分析和程序开发方面的专业知识； (6) 通过与加州大学洛杉矶分校的转基因核心相互作用而产生特定基因突变的转基因动物。