CORE--PROTEOMICS RESOURCE

核心--蛋白质组学资源

• 批准号: 7069765
• 负责人: HELEN KIM
• 金额: $ 11.92万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-30 至 2010-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7069765
• 关键词: animal tissue; autosomal recessive trait; biomedical facility; human tissue; mass spectrometry; molecular biology information system; polycystic kidney; proteomics; statistics /biometry

This Core will provide proteomics technology and support to the investigators in this proposed polycystic kidney disease (PKD) research group. While specific genes and mutations in the proteins encoded by the genes have been identified as starting points for ARPKD and ADPKD, the mutations affect multiple tissues, and therefore almost certainly multiple proteins other than the primary ones encoded by the mutated gene. Proteomics technology allows analysis of patterns of protein changes downstream of a primary genetic defect; we will utilize the resources and personnel of the existing DAB Comprehensive Cancer Center (CCC) Proteomics/Mass Spectrometry Shared Facility to provide proteomics and mass spectrometry capabilities toward the PKD research effort. These efforts will be directed by the existing Shared Facility directors, Drs. Helen Kim and Stephen Barnes, who bring substantial experience in providing such services to the UAB biomedical research community. The rationale for this Core is that systematic proteomic analysis of biological samples from PKD experiments that are hypothesis-driven will enhance the likelihood of identifying previously unidentified proteins or protein modifications that involved in the pathogenesis of the PKD phenotype. Such results can then identify proteins for followup studies, or to which antibodies can be raised for use by the PKD community. An important function of this Core is to provide educational support to the PKD community regarding proteomics technologies and to enable informed utilization of the core technologies by the investigators, particularly for those who have not previously accessed the technologies. These will be in the form of tutorials and/or a workshop, as well as consultation sessions involving the Core directors and individual researchers. PKD this Core services will be enhanced by having a biostatistician working closely with the Core, Dr. Meleth; PKD investigators will be strongly encouraged to consult with Dr.Meleth prior to carrying out an experiment, to insure that various quality control and experimental design issues are addressed. Finally, a research associate will be dedicated to this Core, who will process PKD samples through the appropriate 2D separations and the MS protein identifications. She will coordinate the generation of reports of the final data for each set of samples. This Core will also maintain a database of PKD proteomics data, as part of an ongoing effort by the Shared Facility.

该核心将为拟议的多囊肾病 (PKD) 研究小组的研究人员提供蛋白质组学技术和支持。虽然特定基因和基因编码的蛋白质突变已被确定为 ARPKD 和 ADPKD 的起点，但突变会影响多个组织，因此几乎肯定会影响除突变基因编码的主要蛋白质之外的多种蛋白质。蛋白质组学技术可以分析原发性遗传缺陷下游的蛋白质变化模式；我们将利用现有DAB综合癌症中心（CCC）蛋白质组学/质谱共享设施的资源和人员来提供蛋白质组学和质谱分析 PKD 研究工作的能力。这些工作将由现有共享设施董事 Drs. 指导。 Helen Kim 和 Stephen Barnes 为 UAB 生物医学研究界提供此类服务的丰富经验。该核心的基本原理是，对来自假设驱动的 PKD 实验的生物样本进行系统的蛋白质组学分析，将提高识别先前未识别的参与 PKD 表型发病机制的蛋白质或蛋白质修饰的可能性。然后，这些结果可以识别用于后续研究的蛋白质，或者可以产生抗体以供 PKD 社区使用。该核心的一个重要功能是为以下群体提供教育支持： PKD 社区有关蛋白质组学技术的信息，并使研究人员能够知情地利用核心技术，特别是对于那些以前没有接触过这些技术的人。这些将采取教程和/或研讨会的形式，以及涉及核心主任和个人研究人员的咨询会议。 PKD 这一核心服务将通过与核心密切合作的生物统计学家 Meleth 博士得到加强；强烈鼓励 PKD 研究人员在进行实验之前咨询 Meleth 博士，以确保解决各种质量控制和实验设计问题。最后，一名研究助理将致力于该核心工作，他将通过适当的 2D 分离和 MS 蛋白质鉴定来处理 PKD 样本。她将协调每组样本的最终数据报告的生成。该核心还将维护 PKD 蛋白质组学数据数据库，作为共享设施持续努力的一部分。