Neurochem Chip: Methodology to Study Untethered Rats

Neurochem 芯片：研究不受束缚的大鼠的方法

• 批准号: 7084604
• 负责人: Greg A. Gerhardt
• 金额: $ 41.16万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-30 至 2008-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7084604
• 关键词: acetylcholine; choline; evaluation /testing; extracellular; gamma aminobutyrate; glutamates; laboratory mouse; laboratory rat; microdialysis; microelectrodes; neurochemistry; neurotransmitter transport; technology /technique development; telemetry; wakefulness

DESCRIPTION (provided by applicant): This Stage II CEBRA application involves the development of Neurochem Chip microelectrodes for neurochemical recordings of neurotransmitter release in rats and mice. The long-term goals of this project are to develop Neurochem Chips for rapid in vivo recordings of glutamate, GABA, acetylcholine and choline in the CNS of freely moving rats and mice. In developing the technologies, we will first implement and improve the technologies of Neurochem Chips in rats and mice. First, we plan to optimize the recording approaches in the anesthetized rat and mouse. This will involve microelectrode development in corporation with Thin Film Technologies, Hybrid Circuits, Inc., and Cleveland Medical Devices, Inc. Instrumentation development will occur in cooperation with PCB Assembly, Inc. We will carry out extensive in vitro and in vivo recordings of glutamate, GABA, choline and acetylcholine release and uptake in the prefrontal cortex, striatum and hippocampus of anesthetized rats. There are numerous microelectrode, instrumentation, and recording details that must first be optimized to develop reliable Neurochem Chips. These microelectrodes recordings will be compared with microdialysis measures of glutamate and choline, to try and further validate the techniques. As this grant progresses, the refined and adapted technologies will be applied to studies in awake behaving rats and mice. Freely moving tethered systems will be developed for 4-channel measures in awake rats and 2-channel recordings in mice. In addition, a biotelemetric system will be developed to record from rats with a totally untethered system. This should contribute to 1) increased signal-to-noise of the recordings, 2) increased freedom of movement of the rats, and 3) the potential for monitoring large numbers of rats. The major goal of these studies is to develop Neurochem Chips that are reliable, sensitive and selective for the rapid (second-by-second) determination of neurochemicals in the extracellular space of the brain. This technology could have a major impact on basic science studies of neurotransmitter systems involving drug abuse, disease models and behavior. This CEBRA Stage II mechanism is needed, due to the number of technical issues that must be addressed before these techniques can be disseminated to other researchers.

描述（由申请人提供）：此阶段II CEBRA申请涉及开发用于大鼠和小鼠神经递质释放神经化学记录的神经化学微电极。该项目的长期目标是开发用于在自由移动大鼠和小鼠中枢神经系统中的谷氨酸，GABA，乙酰胆碱和胆碱快速记录的神经化芯片。在开发技术时，我们将首先实施并改善大鼠和小鼠神经化芯片的技术。首先，我们计划优化麻醉大鼠和鼠标中的记录方法。这将涉及薄膜技术，混合电路公司和克利夫兰医疗设备，Inc。仪器开发将与PCB组装合作进行。麻醉大鼠。有许多微电极，仪器和记录细节必须首先优化以开发可靠的神经化学芯片。这些微电极记录将与谷氨酸和胆碱的微透析度量进行比较，以尝试进一步验证这些技术。随着这项赠款的进行，精致和改编的技术将应用于醒着的行为大鼠和小鼠的研究。将开发自由移动的束缚系统，用于在小鼠中清醒大鼠和2通道记录中的4通道措施。此外，将开发一个生物质量系统，以记录具有完全不受限制系统的大鼠。这应该有助于1）记录的信噪比增加，2）增加大鼠运动自由度； 3）监测大鼠的潜力。这些研究的主要目的是开发可靠，敏感和选择性的神经化学芯片，以快速（第二次）确定大脑细胞外空间中的神经化学物质。该技术可能会对涉及滥用药物，疾病模型和行为的神经递质系统的基础科学研究产生重大影响。由于必须解决这些技术，因此需要解决这些技术问题的数量，因此需要这种CEBRA II阶段的机制。