COMPARATIVE MUTATION ACCUMULATION IN RHABDITID NEMATODES

杆状线虫中比较突变积累

基本信息

批准号：
6707062
负责人：
CHARLES F BAER
金额：
$ 2.05万
依托单位：
TRUSTEES OF INDIANA UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2001
资助国家：
美国
起止时间：
2001-03-01 至 2003-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6707062
关键词：
Caenorhabditis elegans Nematoda animal population genetics biochemical evolution gene expression gene mutation molecular genetics species difference

项目摘要

Mutation is the fundamental agent of evolutionary change, and deleterious mutations have been implicated as the causal agent responsible for a variety of important biological phenomena. Recent developments have cast doubt on the prevailing wisdom regarding rates and average effects of new mutations at both the phenotypic and the molecular levels. Unfortunately, good data on mutational properties exist for only two species, Drosophila melanogaster and Caenorhabditis elegans, species that are phylogenetically distant and that differ substantially in life history. Almost nothing is known about how mutational properties vary over fairly small evolutionary distances. Herein I propose to investigate the mutational properties of three species of Rhabditid nematode. Mutations will be allowed to accumulate in initially genetically identical stocks for upwards of 100 generations. Standard methods will be used to estimate the rate and average effect of new mutations with detectable effects on the phenotype, and to partition the variance into within- and among-species components. Molecular methods will also be used to directly estimate the mutation rate at the level of the DNA sequence. The resulting data will allow the interpretation of differences and/or similarities in mutational properties among widely separated taxa in the context of dynamic evolution, that is, within and among closely related species.

突变是进化变化的根本因素，有害突变被认为是导致多种重要生物现象的原因。最近的发展使人们对关于表型和分子水平上新突变的发生率和平均影响的普遍看法产生了怀疑。不幸的是，关于突变特性的良好数据仅存在于两个物种，即果蝇和秀丽隐杆线虫，这两个物种在系统发育上相距较远，并且在生活史上存在很大差异。对于突变特性在相当小的进化距离内如何变化几乎一无所知。在此，我建议研究三种杆状线虫的突变特性。突变将被允许在最初基因相同的种群中积累100代以上。标准方法将用于估计对表型具有可检测影响的新突变的发生率和平均效应，并将方差划分为种内和种间成分。分子方法也将用于直接估计DNA序列水平的突变率。由此产生的数据将允许在动态进化的背景下，即密切相关的物种内部和之间，解释广泛分离的类群之间突变特性的差异和/或相似性。