Identification of Xcv TTSS components

Xcv TTSS 组件的识别

基本信息

批准号：
6584695
负责人：
ALISON J DAVIS
金额：
$ 1.8万
依托单位：
UNIVERSITY OF CALIFORNIA BERKELEY
依托单位国家：
美国
项目类别：
财政年份：
2003
资助国家：
美国
起止时间：
2003-01-01 至 2003-05-10
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6584695
关键词：
Pseudomonadaceae SDS polyacrylamide gel electrophoresis bacteria infection mechanism bacterial proteins cell membrane host organism interaction mass spectrometry membrane proteins plant diseases postdoctoral investigator protein localization protein protein interaction protein purification protein structure function protein transport secretory protein western blottings

项目摘要

DESCRIPTION (provided by applicant): The plant pathogenic bacterium Xanthomonas campestris pv. vesicatoria (Xcv) is the causative agent of bacterial spot disease in tomato and pepper, and requires a functional type III secretion system (TTSS) for its pathogenicity. Gram-negative pathogens of both plants and animals use TTSSs for introducing bacterial effector proteins directly into the host cell cytoplasm. The TTSS apparatus is a multi-component complex composed of proteins conserved in all pathogens and proteins unique to each pathogen. There is little information about the identity or function of plant pathogen-specific proteins required for type 11I secretion. The study of these proteins will provide insight into the initial stages of Xcv pathogenesis. The goal of the research in this proposal is to identify plant pathogen-specific components of the Xcv TTSS. Specifically, the TTSS complex will be biochemically isolated and the constituent proteins identified. Xcv-specific proteins will be characterized by determining their sub-cellular localization and their requirement for protein export from the bacteria and/or protein translocation through the host cell plasma membrane. The function of the Xcv HrpF protein, which is required for translocation of effectors into the plant cell, will also be investigated.

描述（申请人提供）：植物病原菌 Xanthomonas Campestris pv. vesicatoria (Xcv) 是番茄和辣椒中细菌性斑点病的病原体，其致病性需要功能性 III 型分泌系统 (TTSS)。植物和动物的革兰氏阴性病原体都使用 TTSS 将细菌效应蛋白直接引入宿主细胞的细胞质中。 TTSS 装置是一种多组分复合物，由所有病原体中保守的蛋白质和每种病原体特有的蛋白质组成。关于 11I 型分泌所需的植物病原体特异性蛋白的身份或功能的信息很少。对这些蛋白质的研究将有助于深入了解 Xcv 发病机制的初始阶段。本提案的研究目标是鉴定 Xcv TTSS 的植物病原体特异性成分。具体来说，TTSS 复合物将被生化分离并鉴定其组成蛋白。 Xcv特异性蛋白质的特征在于确定它们的亚细胞定位以及它们对从细菌输出蛋白质和/或通过宿主细胞质膜的蛋白质易位的需求。还将研究 Xcv HrpF 蛋白的功能，该蛋白是效应子易位到植物细胞中所必需的。