Identification of Programmed Frameshift Signals in Yeast

酵母中程序化移码信号的鉴定

• 批准号: 7049533
• 负责人: JONATHAN L. JACOBS
• 金额: $ 4.41万
• 依托单位: UNIV OF MARYLAND, COLLEGE PARK
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-15 至 2007-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7049533
• 关键词: Internet; Saccharomyces cerevisiae; bioinformatics; computational biology; computer assisted sequence analysis; computer program /software; computer system design /evaluation; eukaryote; fungal genetics; gene expression profiling; genetic regulatory element; genetic transcription; messenger RNA; microarray technology; molecular biology information system; nucleic acid sequence; ribosomes

DESCRIPTION (provided by applicant): Proof of principle experiments have shown that -1 programmed ribosomal frame shift signals can act as mRNA suicide elements capable of directing messages to the nonsense mediated mRNA decay pathway. Preliminary data has shown that eukaryotic mRNA on which frame shifted ribosomes encounter premature stop codons are subsequently targeted for rapid degradation. Thus, PRF signals may be utilized as a means to post-transcriptionally regulate gene expression by altering the half-life of cellular mRNAs. The proposed research project utilizes bioinformatics methodologies to identify programmed -1 ribosomal frame shift signals from several eukaryotic model organisms. A bioinformatics protocol for the identification PRF signals is in development. Results will be correlated with a series of DNA microarray experiments designed to detect PRF signal containing mRNA transcripts in Saccharomyces cerevisiae. This will provide validation for the bioinformatics results and a resource for refinement of the PRF signal model. A searchable online database will be created for the scientific community to utilize. The proposed studies will serve as the foundation for further research into a newly discovered paradigm of gene regulation.

描述（由申请人提供）： 原理实验证明，-1 编程核糖体移码信号可以充当 mRNA 自杀元件，能够将信息引导至无义介导的 mRNA 衰变途径。 初步数据表明，移码核糖体遇到过早终止密码子的真核 mRNA 随后会被快速降解。因此，PRF信号可用作通过改变细胞mRNA的半衰期来转录后调节基因表达的手段。拟议的研究项目利用生物信息学方法来识别来自几种真核模型生物的编程-1核糖体移码信号。用于识别 PRF 信号的生物信息学协议正在开发中。结果将与一系列 DNA 微阵列实验相关，这些实验旨在检测酿酒酵母中含有 mRNA 转录物的 PRF 信号。这将为生物信息学结果提供验证，并为细化 PRF 信号模型提供资源。将创建一个可搜索的在线数据库供科学界使用。拟议的研究将作为进一步研究新发现的基因调控范式的基础。