Studies of the Cochlear Nucleus Granule Cell Domain

耳蜗核颗粒细胞域的研究

• 批准号: 7139763
• 负责人: DAVID K. RYUGO
• 金额: $ 34.74万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7139763
• 关键词: auditory cortex; auditory discrimination; auditory nuclei; auditory stimulus; axon; brain electrical activity; brain mapping; cats; cerebellum; electron microscopy; granule cell; immunocytochemistry; injection /infusion; laboratory rat; light microscopy; mesencephalon; molecular site; neural information processing; neuronal transport; neurons; pons; radiotracer; sensorimotor system; sound frequency; synaptic vesicles

DESCRIPTION (provided by applicant): Perception of sound is attributed to the pattern of neural activity within the central auditory system. The auditory system is typically defined as a series of structures that are connected directly or indirectly to the cochlea. The significance of a sound, however, depends not only on spectral and temporal characteristics as mediated through the auditory system, but also on the past history and behavioral state of the animal. In other words, sound processing should also include non-auditory systems that would convey sensorimotor functions, emotion, learning, and memory. In this application, we plan to study the nature of non-auditory projections to the cochlear nucleus in order to gain insight into how different systems influence our perception of sound. We propose that the granule cell domain of the cochlear nucleus represents a key site for the integration of mutimodal influences. Preliminary data reveals that somatosensory, vestibular, pontine, reticular, and descending auditory projections access the granule cell domain. We will use anterograde and retrograde tract tracing methods in conjunction with light and electron microscopy to identify projecting neurons and their circuits, immunocytochemical staining procedures to reveal the chemistry of the different terminals, and electrophysiological recording techniques to characterize the response properties of neurons projecting to this region. We will apply nanoparticle technology to explore the use of biodegradable polymers with the goal of creating a new line of neuronal tracers for this study. The possibility of attaching ligands to the surface of the particles could enhance tracer uptake for the selective targeting of specific families of neurons. The data from this research will provide new knowledge on the synaptic organization of a highly integrative structure in the auditory system, and should yield insights into how diverse neural systems shape the coding process for hearing. As we learn about how the somatosensory system modulates hearing, we will gain insight into somatic tinnitus that could contribute to the design of treatment strategies and a better understanding of tinnitus in general.

描述（由申请人提供）：声音的感知归因于中央听觉系统中神经活动的模式。听觉系统通常定义为直接或间接连接到耳蜗的一系列结构。然而，声音的重要性不仅取决于通过听觉系统介导的光谱和时间特征，还取决于动物的过去病史和行为状态。换句话说，声音处理还应包括传达感觉运动功能，情感，学习和记忆的非审计系统。在此应用中，我们计划研究对耳蜗核的非原告预测的性质，以了解不同系统如何影响我们对声音的看法。我们建议人工耳蜗核的颗粒细胞结构域代表了整合肌莫族影响的关键位点。初步数据表明，体感，前庭，丘脑，网状和下降的听觉投影访问颗粒细胞结构域。我们将使用与光和电子显微镜结合使用顺行和逆行痕迹追踪方法来识别投影神经元及其电路，免疫细胞化学染色程序，以揭示不同终端的化学，以及对这种发射物理生理记录的技术的化学，以表征该神经元的响应特性，以表征神经元的响应特性。地区。我们将应用纳米颗粒技术探索可生物降解的聚合物的使用，目的是为这项研究创建新的神经元示踪剂系列。将配体连接到颗粒表面的可能性可以增强示踪剂的摄取，以选择性靶向特定神经元家族。这项研究的数据将提供有关听觉系统中高度整合结构的突触组织的新知识，并应深入了解多样化的神经系统如何塑造听力编码过程。当我们了解体感系统如何调节听力时，我们将深入了解体细胞耳鸣，这可能有助于治疗策略的设计以及对总体上对耳鸣的更好理解。