Unconventional Myosin Distribution Inner Ear Hair Cells

非常规肌球蛋白分布内耳毛细胞

• 批准号: 6786049
• 负责人: ALLISON B COFFIN
• 金额: $ 2.65万
• 依托单位: UNIV OF MARYLAND, COLLEGE PARK
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-07-01 至 2005-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6786049
• 关键词: Agnatha; Chelonia; Crocodilia; Mus musculus; Osteichthyes; Xenopus; chickens; confocal scanning microscopy; ear hair cell; epithelium; gene expression; immunocytochemistry; in situ hybridization; labyrinth; myosins; polymerase chain reaction; predoctoral investigator; protein localization; species difference; transmission electron microscopy; western blottings; zebrafish

DESCRIPTION (provided by applicant): Molecular differences underlie the morphological and physiological diversity seen in inner ear hair cells. This research will use protein distribution to investigate hair cell diversity across vertebrate taxonomic groups. Distribution of unconventional myosins in hair cells will be examined using immunocytochemistry. Confocal microscopy will be used to view fluorescently labeled sensory epithelia using antibodies obtained from either private or commercial sources. Antibody specificity will be verified with western blotting. Gene expression of unconventional myosins will be validated with RT-PCR. These results will be useful for comparative studies of hair cell function, as differences in gene expression or protein distribution may be correlated to functional differences. These results will also be useful to researchers studying the role of unconventional myosins in deafness, as it will open up a wider variety of species for study.

描述（由申请人提供）：分子差异是内耳毛细胞形态和生理多样性的基础。 这项研究将利用蛋白质分布来研究脊椎动物分类群中毛细胞的多样性。将使用免疫细胞化学检查毛细胞中非常规肌球蛋白的分布。共聚焦显微镜将用于使用从私人或商业来源获得的抗体来观察荧光标记的感觉上皮。抗体特异性将通过蛋白质印迹法进行验证。非常规肌球蛋白的基因表达将通过 RT-PCR 进行验证。 这些结果将有助于毛细胞功能的比较研究，因为基因表达或蛋白质分布的差异可能与功能差异相关。这些结果对于研究非常规肌球蛋白在耳聋中的作用的研究人员也将很有用，因为它将开辟更广泛的物种进行研究。