Olfaction in the Lolliguncula brevis

Lolliguncula brevis 的嗅觉

• 批准号: 6998922
• 负责人: Arie Sitthichai Mobley
• 金额: $ 2.85万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-01-01 至 2006-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6998922
• 关键词: biological signal transduction; cyclic AMP; immunocytochemistry; medial olfactory area; neural information processing; neurons; olfactions; olfactory stimulus; predoctoral investigator; receptor expression; squid; voltage /patch clamp

DESCRIPTION (provided by applicant): In general, olfactory receptor neurons (ORNs) are bipolar with an apical dendrite containing cilia or microvilli and a basal unmyelinated axon. In the squid, Lolliguncula brevis, five morphological variations on this general theme have been described. Preliminary data suggests a correlation between morphology and metabolite profiles in squid ORNs. Previous work in our lab has shown that a single squid ORN responds to two odors with opposing currents, suggesting activation of different odorant receptors and signal transduction pathways. Thus, this application is based on the hypotheses that: 1) squid ORN subtypes will have different but overlapping odor specificities; and 2) regardless of ORN subtype, odor-evoked excitatory responses will use a cAMP-mediated transduction pathway. Specific Aim 1 will establish the odor specificities of squid ORN subtypes. Specific Aim 2 will show that odors eliciting a depolarizing response in squid ORN subtypes are transduced by the same cAMP-mediated transduction pathway. Data will be analyzed for correlation between the transduction pathway, ORN metabolite profile, and odor specificities. The sense of smell contributes greatly to quality of life and is important as a warning against spoiled foods, gas leaks, or smoke. Recently, there have been several studies correlating the loss of smell with early onset of Alzheimer's and Parkinson's diseases. These diseases imply that there is a greater need to understand the olfactory system.

描述（由申请人提供）：一般来说，嗅觉受体神经元（ORN）是双极的，具有含有纤毛或微绒毛的顶端树突和基底无髓鞘轴突。在鱿鱼（Lolliguncula brevis）中，已经描述了这一总体主题的五种形态变异。初步数据表明鱿鱼 ORN 的形态和代谢谱之间存在相关性。我们实验室之前的工作表明，单个鱿鱼 ORN 对两种具有相反电流的气味做出反应，表明不同的气味受体和信号转导途径的激活。因此，本申请基于以下假设：1)鱿鱼ORN亚型将具有不同但重叠的气味特异性； 2) 无论 ORN 亚型如何，气味诱发的兴奋反应都将使用 cAMP 介导的转导途径。具体目标 1 将确定鱿鱼 ORN 亚型的气味特异性。具体目标 2 将表明，在鱿鱼 ORN 亚型中引发去极化反应的气味是通过相同的 cAMP 介导的转导途径转导的。将分析数据以了解转导途径、ORN 代谢物谱和气味特异性之间的相关性。嗅觉对生活质量有很大贡献，并且对于防止食物变质、煤气泄漏或烟雾具有重要的警告作用。最近，有几项研究将嗅觉丧失与阿尔茨海默病和帕金森病的早期发作相关联。这些疾病意味着更需要了解嗅觉系统。