Aging, Reperfusion, and Apoptosis:A Proteasome Approach

衰老、再灌注和细胞凋亡：蛋白酶体方法

• 批准号: 6743130
• 负责人: LUKE I. SZWEDA
• 金额: $ 19.47万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-01 至 2005-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6743130
• 关键词: BCL2 gene /protein; Bax gene /protein; SDS polyacrylamide gel electrophoresis; age difference; aging; apoptosis; coronary occlusion /thrombosis; cytochrome c; densitometry; electron microscopy; electrospray ionization mass spectrometry; free radicals; high performance liquid chromatography; laboratory rat; matrix assisted laser desorption ionization; monoclonal antibody; proteasome; reperfusion; western blottings

DESCRIPTION: (provided by applicant) Complications arising from reduction of blood supply to the heart are a leading cause of death and debilitation worldwide. Depending on the duration and severity of the ischemic event, irreparable alterations in cellular homeostasis lead to necrotic cell death. However, reperfusion of viable cardiac tissue can result in cell death by processes unrelated to necrosis. This has been attributed, in part, to increases in the production of oxygen radicals and the induction of programmed cell death, termed apoptosis. The proteasome, a major intracellular proteolytic system, appears to play a critical role in the prevention of apoptosis by degrading certain pro-apoptotic factors. Nevertheless, the proteasome is itself modified by free radical processes and exhibits dramatic declines in activity as a result of coronary occlusion/reperfusion. Furthermore, proteasome expression and specific activity decrease as a function of age. The objectives of the proposed research are to establish a mechanistic link between age and free radical induced loss in proteasome function and stimulation of the apoptotic process during coronary occlusion/reperfusion. Utilizing a physiologically relevant in vivo rat model of coronary occlusion and reperfusion this study seeks to: 1) Identify alterations in proteasome activity; 2) Defme free radical processes which result in loss in proteasome activity; 3) Characterize the progression of apoptosis; and 4) Establish mechanisms by which proteasome inactivation enhances apoptosis. In each aim, the effects of duration of occlusion and reperfusion and age of the animal will be assessed. Thus, these studies will establish biochemical mechanisms by which proteasome function is altered and when, in the sequence of coronary occlusion and reperfusion, critical oxidative events occur. Furthermore, the role of proteasome inactivation in the induction of apoptosis will be established. Finally, the contribution of age-dependent declines in proteasome activity to the progression and extent of coronary occlusion/reperfusion induced apoptosis will be elucidated. Results of the proposed study will therefore define molecular events which are likely to impact the long term progression of heart disease and indicate efficient strategies for favorably influencing the outcome.

描述：（由申请人提供）减少所引起的并发症 心脏的血液供应是死亡和虚弱的主要原因 全世界。根据缺血事件的持续时间和严重程度， 细胞稳态的不可挽回的改变导致坏死性细胞死亡。 然而，活心脏组织的再灌注可导致细胞死亡 与坏死无关的过程。这部分归因于 氧自由基产生的增加和程序化的诱导 细胞死亡，称为细胞凋亡。蛋白酶体是一种主要的细胞内蛋白水解酶 系统，似乎在预防细胞凋亡中发挥着关键作用 降解某些促凋亡因子。然而，蛋白酶体本身 被自由基过程改变并表现出活性急剧下降 由于冠状动脉闭塞/再灌注。此外，蛋白酶体 表达和比活性随着年龄的增长而降低。目标 拟议的研究的目的是建立年龄和年龄之间的机械联系 自由基导致蛋白酶体功能丧失并刺激 冠状动脉闭塞/再灌注期间的细胞凋亡过程。利用一个 冠状动脉闭塞的生理相关的体内大鼠模型和 再灌注 本研究旨在：1) 识别蛋白酶体的改变 活动; 2) 定义导致蛋白酶体损失的自由基过程 活动; 3）表征细胞凋亡的进展； 4) 建立 蛋白酶体失活增强细胞凋亡的机制。在每一个目标中， 闭塞和再灌注持续时间以及动物年龄的影响 进行评估。因此，这些研究将建立生化机制 蛋白酶体功能发生改变时，按照冠状动脉闭塞的顺序 和再灌注时，会发生关键的氧化事件。此外，作用 蛋白酶体失活在诱导细胞凋亡中的作用将被确立。 最后，年龄依赖性蛋白酶体活性下降对 冠状动脉闭塞/再灌注诱导的细胞凋亡的进展和程度 将被阐明。因此，拟议研究的结果将定义 可能影响心脏长期进展的分子事件 疾病并指出有利于影响疾病的有效策略 结果。