In Vitro Macrophage Response to Therapeutic Ultrasound

体外巨噬细胞对治疗性超声的反应

• 批准号: 7081429
• 负责人: THOMAS T TURNER
• 金额: $ 3.04万
• 依托单位: VIRGINIA COMMONWEALTH UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-01 至 2007-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7081429
• 关键词: bioimaging /biomedical imaging; biomarker; cell proliferation; cytokine; fibroblasts; gene expression; inflammation; leukocyte activation /transformation; macrophage; predoctoral investigator; tissue /cell culture; ultrasound biological effect; ultrasound therapy; wound healing

DESCRIPTION (provided by applicant): The overall objective of this research proposal is to evaluate the hypothesis that therapeutic ultrasound (TUS) impacts the healing process by modulating macrophage activity. The specific aims of this project are to: 1) Evaluate cytokine expression and deposition in a cell culture model using macrophages exposed to calibrated doses of TUS at frequencies of 1MHz and 3MHz and 2) Evaluate altered cytokine gene expression in macrophages following exposure to calibrated TUS doses at frequencies of 1MHz and 3MHz. To address these specific aims, I will utilize cultured macrophages treated with TUS in vitro and then assay the conditioned media for various markers of response, i.e. cytokine release into the media, and fibroblast proliferation and neutrophil apoptosis induced by the conditioned media. Also, I will investigate changes in cytokine gene expression following exposure to calibrated TUS doses. Gene expression data will be compared with cytokine release data in an effort to delineate whether different TUS treatment frequencies and doses affect release of preformed substances or induce gene upregulation and subsequent protein release.

描述（由申请人提供）：本研究计划的总体目标是评估治疗性超声 (TUS) 通过调节巨噬细胞活性影响愈合过程的假设。该项目的具体目标是：1) 使用暴露于频率为 1MHz 和 3MHz 的校准剂量 TUS 的巨噬细胞评估细胞培养模型中的细胞因子表达和沉积，2) 评估暴露于校准 TUS 后巨噬细胞中细胞因子基因表达的改变频率为 1MHz 和 3MHz 的剂量。为了实现这些具体目标，我将利用体外经 TUS 处理的培养巨噬细胞，然后测定条件培养基中的各种反应标志物，即细胞因子释放到培养基中，以及条件培养基诱导的成纤维细胞增殖和中性粒细胞凋亡。此外，我还将研究暴露于校准 TUS 剂量后细胞因子基因表达的变化。将基因表达数据与细胞因子释放数据进行比较，以阐明不同的 TUS 治疗频率和剂量是否影响预先形成的物质的释放或诱导基因上调和随后的蛋白质释放。