Group E Sox Regulation of Inner Ear Development

Sox E 组对内耳发育的调节

基本信息

批准号：
7113821
负责人：
KIMBERLY M JAFFE
金额：
$ 2.82万
依托单位：
NORTHWESTERN UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2005
资助国家：
美国
起止时间：
2005-09-01 至 2008-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7113821
关键词：
DNA binding protein SDS polyacrylamide gel electrophoresis Xenopus cell differentiation congenital deafness developmental genetics developmental neurobiology gene mutation labyrinth neurogenesis neuroregulation posttranslational modifications predoctoral investigator protein protein interaction protein structure function transcription factor yeast two hybrid system

项目摘要

DESCRIPTION (provided by applicant): The SOX family of transcription factors belongs to the High Mobility Group (HMG) box superfamily of DMA-binding proteins and regulates many developmental processes, such as cell specification and organ development. The SOX family, comprised of subgroups A-J, is characterized by a highly conserved HMG domain, the DNA binding domain. Two members of SOX family subgroup E, Sox9 and Sox10, are further distinguished by the presence of two uncharacterized homology domains, termed E1 and E2. Both Sox9 and Sox10 have been implicated in inner ear development, and specific mutations in these genes can cause congenital deafness. The aim of this application is to investigate the functional and mechanistic role that these two SOX family members play in the development of the otic placode. This will be explored by looking at potential functional redundancy between Sox9 and Sox10, different protein-protein interactions, and possible post-translational modifications.

描述（由申请人提供）：转录因子的Sox家族属于DMA结合蛋白的高迁移率组（HMG）框，并调节许多发育过程，例如细胞规范和器官发育。由亚组A-J组成的Sox家族的特征是高度保守的HMG结构域DNA结合域。 Sox家族亚组，Sox9和Sox10的两个成员通过两个未表征的同源域（称为E1和E2）进一步区分。 Sox9和Sox10都涉及内耳的发育，这些基因的特定突变可能会引起先天性耳聋。该应用的目的是研究这两个Sox家族成员在Otot placode的发展中起的功能和机械作用。通过查看Sox9和Sox10之间的潜在功能冗余，不同的蛋白质 - 蛋白质相互作用以及可能的翻译后修饰，可以探索这一点。