Investigating cAMP in Taste Transduction

研究 cAMP 在味觉传导中的作用

• 批准号: 7156456
• 负责人: Craig D Roberts
• 金额: $ 3.41万
• 依托单位: UNIVERSITY OF MIAMI SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-06-01 至 2008-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7156456
• 关键词: adenylate cyclase; calcium flux; cyclic AMP; enzyme activity; enzyme inhibitors; fluorescence resonance energy transfer; fluorescent dye /probe; food flavor; immunocytochemistry; inhibitor /antagonist; intermolecular interaction; isozymes; laboratory mouse; laboratory rat; molecular /cellular imaging; neural information processing; phosphodiesterases; predoctoral investigator; second messengers; single cell analysis; stimulant /agonist; stimulus /response; taste; taste buds

DESCRIPTION (provided by applicant): There is unequivocal evidence from biochemistry and physiology that the diffusible second messenger 3'- 5'cyclic adenosine monophosphate (cAMP) is involved in taste transduction. In contrast, recent reports have emphasized the role of Ca2+ signaling in taste transduction (i.e. Receptor --> G protein --> PLCbeta2 --> delta[Ca2+]i). The significance of the original cAMP data is presently unresolved. The goal of my research is to re-investigate cAMP in taste transduction in the light of current understanding. I propose to image real-time changes in intracellular cAMP using novel, genetically-encoded cAMP reporters. These FRET-based reporters permit one to measure single-cell cAMP levels with excellent spatial and temporal resolution. This work will help define taste signaling at the single cell level and enhance our understanding of how gustatory information is processed and coded within taste buds. Specifically, I postulate that cAMP levels in subsets of individual taste receptor cells are altered in response to gustatory stimuli and that cAMP changes are mutually interactive with Ca2+ fluctuations.

描述（由申请人提供）：有来自生物化学和生理学的明确证据表明，可扩散的第二信使3'-5'- 5'- 5'- 5'- 5'-循环腺苷一磷酸（CAMP）参与了味觉转导。相反，最近的报道强调了Ca2+信号传导在味觉转导中的作用（即受体 - > G蛋白 - > plcbeta2-> delta [Ca2+] i）。目前尚未解决原始营地数据的重要性。我的研究的目的是鉴于当前的理解，重新调查训练营。我建议使用新颖的，遗传编码的营地记者对细胞内营地进行实时变化。这些基于FRET的记者允许使用出色的空间和时间分辨率测量单细胞营地水平。这项工作将有助于在单细胞水平上定义味觉信号，并增强我们对味蕾中味觉和编码方式的理解。具体而言，我假设单个味觉受体细胞子集的cAMP水平会因味觉刺激而改变，并且cAMP变化与Ca2+波动相互互动。