The Role of BDNF in Gustatory Neuron Development

BDNF 在味觉神经元发育中的作用

• 批准号: 7158011
• 负责人: Danielle E Harlow
• 金额: $ 2.76万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至 2008-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7158011
• 关键词: Urodela; biomarker; brain derived neurotrophic factor; cell death; cell differentiation; confocal scanning microscopy; cranial nerves; developmental neurobiology; ectoderm; embryogenesis; fluorescence microscopy; ganglions; gene deletion mutation; genetically modified animals; laboratory mouse; mammalian embryology; molecular genetics; nervous system transplantation; neural crest; neural plate /tube; neurogenesis; neurons; nonmammalian vertebrate embryology; predoctoral investigator; somesthetic sensory cortex; taste

DESCRIPTION (provided by applicant): This application investigates the early embryonic events that underlie the development of sensory neurons that innervate the taste system. Specifically, it asks how neurons segregate to gustatory or non-gustatory (somatosensory) modalities. Cranial nerve ganglia that carry taste information arise embryonically from epibranchial placodes and the neural crest. Cell labeling and tissue transplantation techniques will be used to fate map placodes and crest to determine the embryonic origin of gustatory neurons. Gustatory neurons are dependent on brain derived neurotrophic factor (BDNF) for survival. When during development do gustatory neurons require BDNF and what is the source of BDNF required by gustatory neurons? These questions will be addressed using a molecular genetic approach, in which BDNF is selectively deleted from subsets of cranial ganglionic neurons in transgenic mice. The mechanisms involved in cranial nerve development are likely to be broadly involved in many aspects of early sensory system development. My results will lend basic insight into events of normal vertebrate embryogenesis and provide elementary data for the study of neurological defects.

描述（由申请人提供）：本申请研究的早期胚胎事件是支配味觉系统的感官神经元发展的基础。具体而言，它询问神经元如何分离味觉或非闻（体感）方式。带有味觉信息的颅神经神经节是由e骨的姿势和神经rest产生的。细胞标记和组织移植技术将用于命运地图位置和波峰，以确定味觉神经元的胚胎起源。味觉神经元取决于脑衍生的神经营养因子（BDNF）的生存。在发育过程中，味觉神经元是否需要BDNF，而味觉神经元所需的BDNF来源是什么？这些问题将使用分子遗传学方法来解决，其中BDNF从转基因小鼠的颅神经节神经元子集选择性删除。颅神经发育涉及的机制可能广泛参与早期感觉系统发展的许多方面。我的结果将为正常脊椎动物胚胎发生事件提供基本洞察力，并为神经系统缺陷的研究提供基本数据。