Ion transport and motility in larval mosquito stomach

蚊子幼虫胃中的离子运输和运动

• 批准号: 7142172
• 负责人: David Franklin Moffett
• 金额: $ 36.1万
• 依托单位: WASHINGTON STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-06-01 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7142172
• 关键词: cations; ion transport; stomach

DESCRIPTION (provided by applicant): The most prominent organ of larval mosquitoes is the stomach, which makes up the major part of the gastrointestinal tract. This organ is of prominent importance for ionic, volume and acid/base homeostasis. The anterior stomach actively secretes alkali equivalents, generating a compartment of extremely high pH which is of importance for digestion, but also for the susceptibility to biological control agents such as Bacillus thuringienis endotoxin. In the posterior stomach the pH returns to almost neutral values by recovery of alkali equivalents. Most likely, this cycle involves a high turnover of acid/base equivalents as well as of sodium and chloride ions. The processes of ion transport, their regulation and their coordination with peristalsis in the stomach of larval mosquitoes are poorly understood. The project is directed at understanding these processes in two mosquito species: the yellow fever mosquito (Aedes aegypti) and the malaria mosquito of subsaharan Africa Anopheles gambiae. Specifically we will determine: 1. the ion transport mechanisms involved in alkalization of the anterior stomach. 2. the ion transport mechanisms involved in reacidification of the posterior stomach. 3. the mechanisms of regulation of ion transport in the anterior and posterior stomach. 4. the mechanisms of coordination of ion transport with peristalsis in the anterior and posterior stomach. The studies will be carried out, using mainly five different methodological approaches: 1.) Electrophysiological characterization of ion transport across isolated stomach segments and its regulation, using conventional transepithelial techniques and microelectrodes 2.) Analyses of luminal pH changes with isolated stomach segments, using pH indicators and/or pH sensitive microelectrodes in the perfusate 3.) Histochemical and immunhistochemical localization of involved transporters and regulative units/systems 4.) Biochemical determination of transporter activities and their regulation by hormonal/non-hormonal factors 5.) Analyses of peristalsis and its regulation with electrophysiological techniques and image analyses. The results of this project will provide information about specific targets for the development of mosquito control agents.

描述（由申请人提供）：幼虫蚊子的最突出器官是胃，构成了胃肠道的主要部分。该器官对于离子，体积和酸/碱稳态至关重要。胃前胃积极分泌碱等效物，产生极高的pH值，这对于消化至关重要，并且对于对生物控制剂（如黄绿素化蛋白毒素）的敏感性也很重要。在后胃中，pH值通过碱性当量的恢复而恢复到几乎中性的值。最有可能的是，该循环涉及酸/碱等效物以及钠和氯离子的高离职率。离子运输的过程，它们的调节和与幼虫蚊子胃中的蠕动的协调知之甚少。该项目旨在理解两个蚊子中的这些过程：黄热病蚊子（埃德斯埃及）和亚斯萨哈拉非洲亚群岛的疟疾蚊子。具体而言，我们将确定：1。与前胃碱化有关的离子传输机制。 2。与后胃重新配置有关的离子传输机制。 3。在胃前和后胃中离子转运的调节机制。 4。离子转运与蠕动在胃部和后胃中的机制。研究将使用主要使用五种不同的方法学方法进行：1。）使用传统的跨层型技术和微电脱发的传统旋转型技术和微电pH的分析，分析使用分离的胃部段，使用pHSINDICATION和/PH SINSTICS MICRECTICERSHISTIC 3.），使用传统的tansepitherial技术和微pH变化，将腔内pH的变化进行分析，将离子pH的变化和/pH敏感的MICRECHERS HISTRORCHERS 3.）分析，将离子pH的传统旋转型技术和微pH变化进行分析，将离子的胃部及其调节及其调节进行进行。涉及转运蛋白和调节单位/系统的定位4.）生化测定转运蛋白活性及其通过激素/非荷尔蒙因子的调节5.）蠕动分析及其对电生理技术和图像分析的调节。该项目的结果将提供有关蚊子控制剂开发的特定目标的信息。