Electrospun Collagen Type II: Cartilage Feasibilty

静电纺丝 II 型胶原蛋白：软骨可行性

• 批准号: 6873102
• 负责人: Gary L Bowlin
• 金额: $ 17.5万
• 依托单位: VIRGINIA COMMONWEALTH UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-01 至 2006-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6873102
• 关键词: SDS polyacrylamide gel electrophoresis; animal tissue; articular cartilage; bioengineering /biomedical engineering; biomaterial development /preparation; biomechanics; cartilage metabolism; cell line; cell proliferation; chondrocytes; clinical research; collagen; extracellular matrix; human tissue; mucopolysaccharides; nanotechnology; osteoarthritis; tissue /cell culture; tissue engineering; tissue support frame; western blottings

DESCRIPTION (provided by applicant): Osteoarthritis (OA), also known as degenerative joint disease, is one of the most common types of arthritis and is characterized, or associated, with the breakdown of the extracellular matrix (ECM) of articular cartilage. This deterioration of the articular cartilage ECM leads to bone exposure and subsequent bone-on-bone rubbing and associated inflammation that causes pain and loss of movement in the joint. According to the Arthritis Foundation, OA affects an estimated 21 million people in the United States alone with up to 80% of those individuals reporting some limitation in movement or activity. This costs the United Sates economy an estimated $65 billion per year in direct expenses and lost wages/production. Collagen is the natural scaffolding found in all tissues and has been explored extensively for use as a tissue-engineering scaffold with limited success. In this feasibility study, the electrospinning of collagen type II and subsequent chondrocyte seeding will be investigated for potential use in cartilage tissue engineering (Patents Pending). A preliminary of study electrospun collagen type II demonstrated that collagen type II could be electrospun to form non-woven fibrous mats composed of fibers that ranged from 110 nm to 1.8 microns in diameter. The preliminary chondrocyte seeding demonstrated that electrospun collagen type II scaffolds support cell growth and are readily infiltrated. Furthermore, detailed, feasibility studies are now proposed herein. Specific Aim 1 will be collagen type II preliminary processing from bovine articular cartilage including extraction of collagen and purification. Specific Aim 2 will be to electrospin various structures of randomly orientated collagen type II and create a diversity of physical and mechanical characteristics. Specific Aim 3 will characterize the biomechanical/structural properties of the electrospun collagen type II constructs (dry and hydrated) by tensile testing of the bulk material as well as measuring fiber diameter, pore dimensions, scaffold permeability and porosity. Specific Aim 4 will evaluate the collagen type II composition and overall structural properties (fiber diameter and pore size) on the chondrocytes response and overall tissue development after optimum cell seeding and 2, 4 and 6 week interactions with the collagen type II.

描述（由申请人提供）： 骨关节炎 (OA)，也称为退行性关节病，是最常见的关节炎类型之一，其特征是或与关节软骨细胞外基质 (ECM) 的破坏有关。关节软骨 ECM 的恶化会导致骨骼暴露以及随后的骨骼与骨骼之间的摩擦以及相关的炎症，从而导致关节疼痛和运动丧失。据关节炎基金会称，仅在美国就有 2100 万人受到 OA 的影响，其中高达 80% 的人表示运动或活动受到一定限制。据估计，这给美国经济每年带来 650 亿美元的直接开支和工资/生产损失。胶原蛋白是在所有组织中发现的天然支架，并且已被广泛探索用作组织工程支架，但取得的成功有限。在这项可行性研究中，将研究 II 型胶原蛋白的静电纺丝和随后的软骨细胞接种在软骨组织工程中的潜在用途（正在申请专利）。对电纺 II 型胶原蛋白的初步研究表明，II 型胶原蛋白可以通过电纺丝形成由直径范围为 110 nm 至 1.8 微米的纤维组成的非织造纤维垫。初步的软骨细胞接种表明，电纺 II 型胶原蛋白支架支持细胞生长并且易于渗透。此外，本文现在提出了详细的可行性研究。具体目标1是对牛关节软骨进行II型胶原蛋白的初步加工，包括胶原蛋白的提取和纯化。具体目标 2 是静电纺丝随机取向的 II 型胶原蛋白的各种结构，并产生多种物理和机械特性。具体目标 3 将通过散装材料的拉伸测试以及测量纤维直径、孔径、支架渗透性和孔隙率来表征电纺 II 型胶原蛋白构建体（干燥和水合）的生物力学/结构特性。具体目标 4 将在最佳细胞接种以及与 II 型胶原蛋白相互作用 2、4 和 6 周后评估 II 型胶原蛋白的组成和整体结构特性（纤维直径和孔径）对软骨细胞反应和整体组织发育的影响。