Molecular Mechanism of Acupuncture

针灸的分子机制

• 批准号: 7014021
• 负责人: GABRIELA GARCIA
• 金额: $ 18.31万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-02-15 至 2009-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7014021
• 关键词: RNase protection assay; acupuncture /acupressure; alternative medicine; analgesia; biological signal transduction; blood tests; cell adhesion molecules; chemokine; cholinergic receptors; cytochrome P450; cytokine; electrostimulus; enzyme linked immunosorbent assay; gene expression; genetic regulation; glomerulonephritis; immunocytochemistry; immunoprecipitation; inflammation; intermolecular interaction; laboratory rat; molecular biology; nonhuman therapy evaluation; urinalysis; vasodilation; western blottings

DESCRIPTION (provided by applicant): Meridian medicine, such as acupuncture, acupressure, and Qigong, have been commonly used to treat a variety of diseases and disorders; however, the potency of these alternative methods has not been widely accepted by mainstream due to a lack of scientific evidence. Although substantial evidences have been accumulated that acupuncture has prominent analgesic effect; there is no systemic research regarding signaling pathways mediated by acupuncture in treating diseases. Recently, we have found that acupuncture treatment lead to significant anti-inflammatory as well as vasodilatory modulation in animal models. We hypothesize that acupuncture analgesia might function partially by its direct and/or indirect inhibition of inflammation, since pain is frequently associated with inflammation and acupuncture has been successfully used to treat a number of human inflammatory diseases. We have also found that cholinergic pathway may play an important role in acupuncture-mediated anti-inflammatory effects and other therapeutic benefits in the animal model as demonstrated by microarray using a rat oligo set containing 6,000 probes. The purposes of this proposal are 1), to further determine cellular and molecular mechanisms by which acupuncture attenuates inflammation in vivo in rats; and 2), to identify the signaling pathways by which acupuncture mediates anti-inflammatory and vasodilatory effects in the animal model. This study represents an unexplored area of research and would provide important insight into how acupuncture technology can be linked to modern concept of gene regulation in biological systems and may further offer a feasible strategy to increase understanding the molecular biology of alternative medicine in meridian therapies. We believe that our studies may open new era towards molecular understanding of acupuncture treatment in pain, inflammation, as well as a variety of vascular diseases in humans.

描述（由申请人提供）：针灸，启动和气功等子午医学通常用于治疗各种疾病和疾病；但是，由于缺乏科学证据，主流尚未被主流所接受的这些替代方法的效力。尽管已经积累了大量证据，即针灸具有显着的镇痛作用。关于针灸治疗疾病介导的信号通路没有系统性研究。 最近，我们发现针灸治疗导致动物模型中的显着抗炎和血管舒张调节。我们假设针灸镇痛可能因其直接和/或间接抑制炎症而部分发挥作用，因为疼痛经常与炎症和针灸有关，已成功地用于治疗许多人类炎症性疾病。我们还发现，胆碱能途径在针灸介导的抗炎作用和动物模型中的其他治疗益处中可能起着重要作用，如微阵列使用含有6,000探针的大鼠寡核能表所证明的那样。该提案的目的是1），以进一步确定针灸减轻大鼠体内炎症的细胞和分子机制；和2），以确定针灸在动物模型中介导抗炎和血管舒张作用的信号传导途径。这项研究代表了一个未开发的研究领域，将提供重要的见解，即如何将针灸技术与生物系统中的基因调节的现代概念联系起来，并可能进一步提供可行的策略，以增加理解子午治疗中替代医学的分子生物学。我们认为，我们的研究可能会使对针灸治疗的分子理解疼痛，炎症以及人类的各种血管疾病开放。