Healing wounds with medicinal yeast peptides

用药用酵母肽治愈伤口

• 批准号: 6881757
• 负责人: DONNA J SCHLEMM
• 金额: $ 10万
• 依托单位: LOTUS GROUP, INC.
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-03-01 至 2006-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6881757
• 关键词: Saccharomyces cerevisiae; affinity chromatography; biotechnology; biotherapeutic agent; cell migration; cell proliferation; chimeric proteins; clinical research; drug design /synthesis /production; drug screening /evaluation; fungal proteins; growth factor; keratinocyte; laboratory mouse; molecular cloning; polymerase chain reaction; protein purification; protein quantitation /detection; protein reconstitution; protein sequence; skin disorder chemotherapy; topical drug application; transfection /expression vector; two dimensional gel electrophoresis; western blottings; wound healing

DESCRIPTION (provided by applicant): There are many illnesses and conditions which occur in elderly populations which disrupt tissue repair such as pressure ulcers, atherosclerosis, diabetes mellitus, persistent bacterial and fungal infections, and malignancies. In general, wound healing in an aging individual occurs at a slower rate than a younger individual. This slowing process observed at the tissue and cellular level is manifested by reduced oxygen consumption, slower glucose metabolism, slower inflammatory response, reduced macrophage function and fibroblast responses and slower capillary growth and re-epithelialization rates. Although several studies have demonstrated that the addition of growth factors or small peptides can reduce the time required for wound closure, there is still not an adequate topical remedy in the marketplace. The research proposed in this investigation seeks to discover the identity and chemistry behind the efficacy of old medications which use alcoholic extracts of yeast as their active ingredient. Experiments using two dimensional electrophoresis and mass spectroscopy are proposed in the first phase of this research to identify the amino acid sequences of the yeast peptides recovered from the medication. Published experiments demonstrate that these peptides accelerate healing in normal and diabetic mice. The genes that encode these peptides will then be used to construct yeast expression vectors that over-express and secrete on demand pure proteins. The proteins in turn will be immunoaffinity purified and tested later for their biological activity in established animal models for wound healing. The Lotus Group, Inc. intends to formulate a medication based on the results, manufacture this medication and bring it to the marketplace.

描述（由申请人提供）：在老年人群中发生了许多疾病和状况，这些疾病和状况破坏了组织修复，例如压溃疡，动脉粥样硬化，糖尿病，糖尿病，持续性细菌和真菌感染以及恶性肿瘤。通常，衰老个体的伤口愈合的发生率比年轻人慢。在组织和细胞水平上观察到的这种放缓的过程通过降低的氧气消耗，葡萄糖代谢较慢，炎症反应较慢，巨噬细胞功能降低，巨噬细胞功能和成纤维细胞反应以及较慢的毛细管生长以及重新上皮率较慢。尽管一些研究表明，增加生长因子或小肽可以减少伤口闭合所需的时间，但市场上仍然没有足够的局部补救措施。这项研究中提出的研究旨在发现旧药物的疗效背后的身份和化学，这些药物使用酵母的酒精提取物作为其活性成分。在本研究的第一阶段提出了使用二维电泳和质谱法的实验，以鉴定从药物中回收的酵母肽的氨基酸序列。已发表的实验表明，这些肽在正常和糖尿病小鼠中加速了愈合。然后，编码这些肽的基因将用于构建过表达和分泌纯蛋白的酵母表达载体。蛋白质反过来将进行免疫亲和力纯化，然后在既定的动物模型中进行生物学活性进行免疫亲和力，以进行伤口愈合。 Lotus Group，Inc。打算根据结果制定药物，生产这种药物并将其带入市场。