Lactoferrin prevents biofilm formation at wound

乳铁蛋白可防止伤口处生​​物膜的形成

• 批准号: 6933304
• 负责人: NING HUANG
• 金额: $ 10万
• 依托单位: VENTRIA BIOSCIENCE
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-06-01 至 2006-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6933304
• 关键词: Pseudomonas aeruginosa; Staphylococcus aureus; affinity chromatography; antibacterial agents; antibiotics; bacterial disease; biofilm; biotechnology; biotherapeutic agent; disease /disorder prevention /control; drug resistance; drug screening /evaluation; enzyme linked immunosorbent assay; genetically modified plants; growth inhibitors; high throughput technology; lactoferrin; lysozyme; peptide chemical synthesis; protein purification; recombinant proteins; rice; wound healing

DESCRIPTION (provided by applicant): Wound healing is a major health issue for the elderly. Biofilm development at the wound site renders many existing treatments ineffective in treating wound pathogens because when bacteria is protected by biofilm it increases its resistance to antibiotics. Bacteria protected by biofilm demonstrate a 100 to 1000 fold increase in resistance to antibiotics. Recent research has shown that human lactoferrin is effective in preventing biofilm formation. Production of recombinant human lactoferrin (rhLF) in rice grain provides the foundation for large quantities of raw material at an affordable cost. A rhLF-based treatment to enhance wound healing is proposed. In phase I, rice grain will be scaled up and a process to efficiently purify lactoferrin from rice grain will be developed. This procedure will be used to prepare quality rhLF, which would then be used to test rhLF's effect on biofilm formation for various bacterial strains isolated from wound site including antibiotic resistant strains.

描述（由申请人提供）：伤口愈合是老年人的主要健康问题。伤口部位生物膜的形成使得许多现有的治疗方法无法有效治疗伤口病原体，因为当细菌受到生物膜保护时，它会增加其对抗生素的抵抗力。受生物膜保护的细菌对抗生素的耐药性增加了 100 至 1000 倍。最近的研究表明，人乳铁蛋白可有效防止生物膜形成。在米粒中生产重组人乳铁蛋白 (rhLF) 为以可承受的成本生产大量原材料奠定了基础。提出了一种基于 rhLF 的治疗方法来增强伤口愈合。第一阶段将扩大米粒规模，并开发一种从米粒中有效纯化乳铁蛋白的工艺。该程序将用于制备优质 rhLF，然后用于测试 rhLF 对从伤口部位分离的各种细菌菌株（包括抗生素耐药菌株）生物膜形成的影响。