EVOLUTIONARY GENETICS OF MALE REPRODUCTIVE TRAITS IN MUS

MUS 雄性生殖特征的进化遗传学

基本信息

批准号：
7068084
负责人：
MATTHEW D DEAN
金额：
$ 4.88万
依托单位：
UNIVERSITY OF ARIZONA
依托单位国家：
美国
项目类别：
财政年份：
2004
资助国家：
美国
起止时间：
2004-05-17 至 2007-05-16
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7068084
关键词：
Mus musculus evolution fertility gene expression genetic library genetic polymorphism genetics genotype male natural selections phenotype postdoctoral investigator reproduction species difference sperm

项目摘要

DESCRIPTION (provided by applicant): A major goal in evolutionary genetics is to connect genotypic and phenotypic variation both within and between species to better understand the functional consequences of molecular variation, and ultimately the genetic basis of traits that affect fitness. Several male reproductive traits in mammals are likely to play a key role in fitness. Recent work in a variety of species has shown that genes involved in male reproduction are often targets of natural selection. The three main objectives of this study are to: 1) Test the hypothesis that genes involved in male reproduction in house mice are subject to positive natural selection, both within and between species. Rates of divergence in candidate loci will be investigated among four closely related Mus lineages and Rattus rattus. Polymorphism will be investigated in a sample of 20 wild-derived M. domesticus sampled from its native range; 2) Document naturally occurring phenotypic variation in sperm competitive ability using these same 20 M. domesticus males. Males will compete against a common male genotype for fertilizations; 3) Connect phenotypic variation in sperm competitive ability to genotypic variation observed in candidate loci. These studies will help uncover the genetic basis of male reproductive fitness in a natural population of house mice.

描述（由申请人提供）：进化遗传学的主要目标是在物种内部和物种之间连接基因型和表型变异，以更好地了解分子变异的功能后果，并最终导致影响适应性的性状的遗传基础。哺乳动物中的几种男性生殖特征可能在健身中起关键作用。在各种物种中的最新工作表明，与男性繁殖有关的基因通常是自然选择的靶标。这项研究的三个主要目标是：1）检验以下假设：室内小鼠中与男性繁殖有关的基因在物种内部和物种之间都受到积极的自然选择。候选基因座的分歧率将在四个密切相关的MUS谱系和拉图斯之间进行研究。将在来自其天然范围的20个野生衍生的家用植物的样本中研究多态性。 2）使用这些相同的20 M.家族雄性，可以证明精子竞争能力的自然存在表型变化。雄性将与普通的男性基因型竞争肥料。 3）将精子竞争能力的表型变异连接到候选基因座中观察到的基因型变异。这些研究将有助于揭示自然小鼠群体中男性生殖健康的遗传基础。