Optical Intrinsic Signal Imaging of Seizure

癫痫发作的光学本征信号成像

基本信息

批准号：
7033958
负责人：
JAMES WY CHEN
金额：
$ 16.14万
依托单位：
UNIVERSITY OF CALIFORNIA LOS ANGELES
依托单位国家：
美国
项目类别：
财政年份：
2002
资助国家：
美国
起止时间：
2002-04-01 至 2008-03-31
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): The development of both structural and functional neuroimaging has progressed significantly in the last decade. However, clinical application of functional neuroimaging techniques in epilepsy has been complicated by their unsatisfactory temporal and spatial resolution. Optical imaging of intrinsic signals (OIS) is a functional neuroimaging technique that measures cortical reflectance changes with millisecond temporal resolution and micron spatial resolution. These optical images are correlated with neuronal activity and are due to changes in cerebral blood volume, light scattering, and in hemoglobin and cytochrome oxidation state. In the preliminary studies, OIS was recorded from the somatosensory cortex of rats at, near infrared frequency (850 nm). OIS was shown to correlate well with seizure activities, in a recoverable and reproducible fashion. It was also noted that OIS changes could precede the initial EEG spikes for up to I minute. The goal of this project is to correlate multiplewavelength optical signals with both electrophysiological and immunohistochemical markers, and to establish a primary foundation of interpreting OIS seizure data. The results of this project can be applied in the future in various epilepsy models, such as in a cortical kindling model for the study of neuronal network behaviors during kindling process. OIS imaging might hold a promising potential of better understanding of epileptogenesis, seizure induction, cessation and propagation pathways. Additional application of OIS imaging during epilepsy surgery in defining the eloquent cortex and confirming the seizure focus should be explored thoroughly in the future. The candidate, Dr. Chen, is a neurologist and a cellular electrophysiologist who has the expertise of using patch clamp technique. His career goal is to elucidate the basic mechanisms of epilepsy by integrating all the research skills that he has learned or will learn in electrophysiology, optical imaging, neurochemistry and computer simulation. This project will be conducted at the UCLA School of Medicine under Drs. Toga and Wasterlain's guidance. Both mentors are internationally known authorities in their respective fields of research.

描述（由申请人提供）：结构和在过去的十年中，功能性神经影像学已经显着发展。但是，功能性神经影像技术在癫痫中的临床应用它们的时间和空间分辨率不令人满意，使其复杂化。内在信号（OIS）的光学成像是功能性神经影像学测量皮质反射率随时间毫秒而变化的技术分辨率和微米空间分辨率。这些光学图像相关具有神经元活性，是由于脑血容量的变化，光散射，在血红蛋白和细胞色素氧化态中。在初步研究，从大鼠的体感皮质中记录了OIS，接近红外频率（850 nm）。 OI被证明与癫痫发作良好相关以可回收和可重现的方式进行活动。还指出 OIS的变化可以在最初的EEG尖峰之前长达I分钟。目标这个项目的是将多波长光学信号与两者相关联电生理和免疫组织化学标记，并建立一个解释OIS癫痫发作数据的主要基础。结果的结果将来可以在各种癫痫模型中应用项目，例如研究神经元网络行为的皮质点燃模型点燃过程。 OIS成像可能具有更好的潜力了解癫痫发生，癫痫诱导，停止和传播途径。在癫痫手术期间OIS成像的其他应用定义雄辩的皮质并确认癫痫发作的重点应该是将来彻底探索。候选人Chen博士是神经科医生以及具有使用斑块夹的专业知识的细胞电生理学家技术。他的职业目标是阐明癫痫的基本机制整合他学到或将学习的所有研究技能电生理学，光学成像，神经化学和计算机模拟。该项目将在DRS的UCLA医学院进行。长袍和Wasterlain的指导。两位导师都是国际知名的当局在各自的研究领域。