Structure & Function of Epididymis and Vas Deferens

结构

• 批准号: 7019172
• 负责人: KENNETH P ROBERTS
• 金额: $ 26.1万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-09-29 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7019172
• 关键词: SDS polyacrylamide gel electrophoresis; acrosome; active sites; biological signal transduction; calcium channel; calcium flux; confocal scanning microscopy; cysteine; egg /ovum; epididymis; fertilization; glycoproteins; immunoprecipitation; laboratory rat; membrane fusion; membrane proteins; phosphorylation; polymerase chain reaction; protein binding; protein structure; protein structure function; receptor; sperm; sperm capacitation; western blottings

DESCRIPTION (provided by applicant): Funds are requested to study the specific functions of the Crisp-1 protein. Crisp-1 is produced in the epididymis and associates with the sperm during transit through the organ. The high similarity to a known ryanodine receptor agonist suggests that Crisp-1 may have a role in ion channel regulation. Changes in calcium concentrations are required for sperm to become capacitated and undergo the acrosome reaction in preparation for fertilization. In vitro capacitation studies of rat sperm show that when Crisp-1 is present, sperm are prohibited from capacitating. The specific aims focus on determining the mechanisms involved in the inhibition of capacitation by Crisp-l, identification of the receptor for Crisp-1 on sperm, identification of the Crisp-1 active site, and investigation of the ability of other members of the Crisp family to mimic the actions of Crisp-1. Crisp-1 will also be tested for its ability to inhibit capacitation and the acrosome reaction in human sperm. Finally, potential actions of Crisp-1 on the egg, such as the ability to regulate cortical granule fusion, will be examined. The proposed studies will provide significant contributions to our understanding of sperm function and may also have potential application to male infertility and contraceptive development.

描述（由申请人提供）：要求资金研究CRISP-1蛋白的特定功能。 CRISP-1是在附加膜中产生的，并在通过器官过境期间与精子相关。与已知的ryanodine受体激动剂的高相似性表明，CRISP-1可能在离子通道调节中起作用。钙浓度的变化是要使精子能够被电容的，并经历了高级反应以准备受精。大鼠精子的体外电容研究表明，当存在CRISP-1时，将禁止精子能力。具体目的是确定Crisp-L，Crisp-1的识别SCRISP-1对精子的受体的识别，CRISP-1活跃部位的识别以及对Crisp家族其他成员模仿Crisp-1的作用的能力的机制。 CRISP-1还将因其抑制电容的能力和人类精子中的Adrosom反应的能力而进行测试。最后，将检查CRISP-1对卵的潜在作用，例如调节皮质颗粒融合的能力。拟议的研究将为我们对精子功能的理解做出重大贡献，并可能对男性不育症和避孕发育有潜在的应用。