Molecular view of transcription activation & repression

转录激活的分子视角

• 批准号: 7030480
• 负责人: Leemor Joshua-Tor
• 金额: $ 30.36万
• 依托单位: COLD SPRING HARBOR LABORATORY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-02-01 至 2010-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7030480
• 关键词: cell differentiation; cell growth regulation; environmental adaptation; fungal genetics; fungal proteins; gene induction /repression; genetic transcription; protein protein interaction; protein structure function; yeasts

DESCRIPTION (provided by applicant): Transcriptional regulation in eukaryotes relies on factors that interact with specific sequences in gene promoters. This is a highly regulated and finely tuned process, which is critical to cell growth, differentiation and development and response to environmental changes. It is largely governed by negative and positive protein-protein interactions between regulatory factors, yet they are poorly understood. Understanding these interactions at the atomic level is key to understanding the mechanism of how transcriptional regulation works. The yeast GAL system, which serves as a paradigm for transcriptional regulation, provides a unique opportunity to examine these interactions at an atomic level, especially in light of the wealth of genetic information available. The objective of this proposal is to study the interactions and molecular characteristics of the factors involved in a transcriptional switch, and to shed light on the characteristics of activation domains, which remain largely enigmatic. Relevance: Transcriptional regulation in eukaryotes is a highly regulated and finely tuned process, which is critical to cell growth, differentiation and development and response to environmental changes. Our objective is to understand how this important type of regulation works on a very detailed atomic level.

描述（由申请人提供）：真核生物中的转录调控依赖于与基因启动子中的特定序列相互作用的因子。这是一个高度调控和精细调整的过程，对于细胞生长、分化和发育以及对环境变化的反应至关重要。它很大程度上受调节因子之间的负向和正向蛋白质-蛋白质相互作用控制，但人们对它们知之甚少。在原子水平上理解这些相互作用是理解转录调控工作机制的关键。酵母 GAL 系统作为转录调控的范例，提供了在原子水平上检查这些相互作用的独特机会，特别是考虑到可用的遗传信息丰富。该提案的目的是研究转录开关中涉及的因子的相互作用和分子特征，并阐明激活域的特征，这些特征在很大程度上仍然是个谜。 相关性：真核生物的转录调控是一个高度调控和精细调整的过程，对于细胞生长、分化和发育以及对环境变化的响应至关重要。我们的目标是了解这种重要的监管类型如何在非常详细的原子层面上发挥作用。