Optical imaging of neural activity in auditory system

听觉系统神经活动的光学成像

• 批准号: 6614294
• 负责人: James A Kaltenbach
• 金额: $ 13.06万
• 依托单位: WAYNE STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-04-01 至 2005-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6614294
• 关键词: auditory nuclei; bioimaging /biomedical imaging; electrophysiology; hamsters; imaging /visualization /scanning; neural conduction; neural information processing; neuroimaging; neurons; neurophysiology; optics; sound

DESCRIPTION (provided by applicant): This project will examine the potential of optical imaging technology for the investigation of functional activity on the surface of the dorsal cochlear nucleus (DCN). This technology has recently come of age as a result of a new generation of optical imaging cameras characterized by ultra high spatial and temporal resolution and the introduction of new, non-toxic voltage sensitive dyes. When these new tools are used in combination, optical imaging provides a powerful approach to mapping brain activity in real time. In the proposed project we will employ a state-of-the-art optical imaging system to study the spatio-temporal dynamics of stimulus-driven and spontaneous activity on the surface of the DCN. First, we will obtain real time image sequences of DCN responses to acoustic stimuli that change in frequency and intensity. The purpose of this experiment is to test the feasibility of optical imaging to examine the spatial and temporal dynamics of information flow within and across the tonotopic array of the DCN. Second, we will use optical imaging to map and quantify increases in spontaneous activity that can be induced in the DCN by intense sound exposure. The goal will be to determine the spatial patterns of this hyperactivity and its temporal behavior. Third, we will image the spatio-temporal patterns of activity that occur on the DCN surface in response to parallel fiber stimulation. The image sequences will be acquired during and after electrical stimulation of parallel fibers at a number of sites across the DCN surface. The goal is to map the path of activation as it flows along the parallel fibers to its target neurons. The significance of these experiments is that they will yield information regarding the dynamics of signal processing and reveal new aspects of DCN circuitry that have been difficult to study electrophysiologically.

描述（由申请人提供）：该项目将研究光学成像技术在研究耳蜗背核（DCN）表面功能活动方面的潜力。由于新一代光学成像相机具有超高空间和时间分辨率以及新型无毒电压敏感染料的引入，这项技术最近已经成熟。当这些新工具结合使用时，光学成像提供了一种实时绘制大脑活动图的强大方法。在拟议的项目中，我们将采用最先进的光学成像系统来研究 DCN 表面刺激驱动和自发活动的时空动力学。首先，我们将获得 DCN 对频率和强度变化的声刺激响应的实时图像序列。本实验的目的是测试光学成像检查 DCN 音调阵列内和跨 DCN 音调阵列的信息流的空间和时间动态的可行性。其次，我们将使用光学成像来绘制和量化 DCN 中因强烈的声音暴露而引起的自发活动的增加。目标是确定这种过度活跃的空间模式及其时间行为。第三，我们将对 DCN 表面响应平行纤维刺激而发生的活动时空模式进行成像。图像序列将在 DCN 表面多个部位的平行纤维电刺激期间和之后采集。目标是绘制沿着平行纤维流向目标神经元的激活路径。这些实验的意义在于，它们将产生有关信号处理动态的信息，并揭示 DCN 电路的新方面，而这些方面很难用电生理学方法进行研究。