Core-Animal Core

核心-动物核心

• 批准号: 6781673
• 负责人: Laureano D Asico
• 金额: $ 9.88万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-12-01 至 2008-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6781673
• 关键词: angiotensin receptor; animal breeding; animal colony; biomedical facility; blood pressure; clinical research; dopamine receptor; familial hypertension; genetically modified animals; laboratory mouse; telemetry

The mice (D1 and D3 receptor mutants and GRK4 wild type and A142V transgenes) required for project by Jose in this PPG will be bred and maintained by the Animal Core. Colonies to support adult mice of 8-14 weeks will be developed from initial breeders. Heterozygous (+/-) mice will be bred to provide homozygous (+/+) wild type, heterozygous (+/-) and homozygous (-/-) deleted mice in the same litter. The Animal Core has in place functioning breeding colonies and has a detailed plan that specifies the number of mice necessary to support the proposed research. The Core has the infrastructure in place to maintain breeding information in a database that catalogues ear tag identification and breeding history and numerous other parameters. The Animal Core will collect blood and/or tail tissue from weanling mice for genotyping in the Analytical Core. The Animal Core will also measure the conscious mean arterial blood pressure directly by radio-telemetry in a subset of each single-gene deleted or transgenic strain. The Animal Core will provide all technical support for all protocols involving animals and assure that the PPG is in compliance with all institutional regulations regarding animal breeding and experimental use.

Jose在该PPG中所需的Project所需的小鼠（D1和D3受体突变体和GRK4野生型和A142V转基因）将由动物核心繁殖和维持。最初的育种者将开发支持8-14周成年小鼠的菌落。杂合（+/-）小鼠将繁殖以提供纯合（+/+）野生型，杂合（+/-）和纯合子（ - / - ）删除的小鼠在同一垫料中。动物核心具有起作用的繁殖菌落，并制定了一个详细的计划，该计划指定了支持拟议研究所需的小鼠数量。核心具有适当的基础设施，可以在数据库中维护繁殖信息，该数据库将EAR TAG标识和育种历史记录以及许多其他参数。这 动物核心将从断奶小鼠中收集血液和/或尾部组织，以进行基因分型 分析核心。动物核心还将在每个单基因删除或转基因菌株的子集中直接测量意识平均动脉血压。动物核心将为涉及动物的所有方案提供所有技术支持，并确保PPG符合有关动物育种和实验用途的所有机构法规。