Patient oriented research in chronic wounds

以患者为导向的慢性伤口研究

• 批准号: 7035112
• 负责人: David Margolis
• 金额: $ 12.09万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-05-01 至 2011-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7035112
• 关键词: Adenoviridae; adult human (21+); biopsy; chronic disease /disorder; clinical trial phase I; combination therapy; compression; cytotoxicity; gene dosage; gene therapy; histology; human therapy evaluation; iatrogenic disease; immunocytochemistry; injection /infusion; leg; outcomes research; patient oriented research; platelet derived growth factor; skin ulcer; statistics /biometry; therapy; transfection /expression vector; wound healing

DESCRIPTION (provided by applicant): David J. Margolis, MD PhD is currently an Associate Professor of Dermatology, an Associate Professor of Epidemiology in the Department of Biostatistics and Epidemiology, and a Senior Scholar (core faculty member) in the Center for Clinical Epidemiology and Biostatistics of the University of Pennsylvania. He is an established patient-oriented researcher with an extensive track record of successful research. His research record attests to his commitment to a career in patient-oriented research, which has been focused on the prevention and treatment of chronic wounds, and his desire to be a mentor. He has applied a wide variety of epidemiologic tools and patient-oriented research methods to address research issues. His immediate and long-term career goals center on his desire and intention to continue to evolve and mature as a patient-oriented researcher, teacher, and mentor. Resources available to him, on and around the Penn campus, include all of the components necessary for successful patient-oriented research and mentoring including biostatistical support, data management support, and state of the art research/clinical laboratory services. His patient-oriented NAIMS-funded research project is, after many years of delay, in its first of five years. Most patients with chronic wounds of the lower extremity fail to heal in a reasonable period of time. We Have hypothesized that a growth factor associated with wound healing PDGF-BB, when produced in large quantities within the wound bed due to adenovirus-mediated gene over expression by the cells of the wound bed, will dramatically enhance wound healing. This study will use a unique and innovative gene therapy approach, adenovirus-Ad5 and the PDGF-B gene, to insure delivery of a cytokine growth factor to a non-healing wound. The aim of the proposed research study is to assess whether PDGF-BB via in vivo PDGF-BB gene transduction via an intra-ulcer injection of H5.020CMVPDGF-B in patients with a chronic venous leg ulcers can induce the local acquisition of endothelial precursor cells.

描述（由申请人提供）：David J. Margolis，医学博士博士，现任皮肤病学副教授、生物统计学和流行病学系流行病学副教授、临床流行病学中心高级学者（核心教员）和宾夕法尼亚大学的生物统计学。他是一位以患者为中心的资深研究人员，拥有丰富的成功研究记录。 他的研究记录证明了他对以患者为导向的研究事业的承诺，该研究的重点是慢性伤口的预防和治疗，以及他成为导师的愿望。他应用了各种流行病学工具和以患者为中心的研究方法来解决研究问题。他的近期和长期职业目标集中在他作为一名以患者为中心的研究员、教师和导师继续发展和成熟的愿望和意图。他在宾夕法尼亚大学校园内和周围可用的资源包括成功以患者为导向的研究和指导所需的所有组成部分，包括生物统计支持、数据管理支持和最先进的研究/临床实验室服务。他的以患者为导向、由 NAIMS 资助的研究项目经过多年的拖延，现在已进入五年的第一年。 大多数下肢慢性伤口的患者无法在合理的时间内愈合。我们假设，与伤口愈合相关的生长因子PDGF-BB，当由于伤口床细胞腺病毒介导的基因过度表达而在伤口床内大量产生时，将显着促进伤口愈合。这项研究将使用一种独特且创新的基因治疗方法，即腺病毒-Ad5 和 PDGF-B 基因，以确保将细胞因子生长因子输送到未愈合的伤口。拟议研究的目的是评估慢性静脉性腿部溃疡患者通过溃疡内注射 H5.020CMVPDGF-B 进行体内 PDGF-BB 基因转导是否可以诱导内皮前体细胞的局部获得细胞。