CORE--NEUROCHEMISTRY

核心--神经化学

• 批准号: 6968948
• 负责人: Holly Marie Moore
• 金额: $ 19.35万
• 依托单位: NEW YORK STATE PSYCHIATRIC INSTITUTE DBA RESEARCH FOUNDATION FOR MENTAL HYGIENE, INC
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-24 至 2009-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6968948
• 关键词: baboons; biomedical facility; brain imaging /visualization /scanning; catecholamines; indoleamine; magnetic resonance imaging; method development; microdialysis; neural transmission; neurochemistry; neurotransmitters; pharmacokinetics; radiotracer; receptor binding; schizophrenia

The Neurochemistry Core will integrate and consolidate the activities performed in several basic laboratories of this Center into three units, under the direction of Drs Moore, Cooper and Javitch, respectively: 1) In vivo and ex vivo determination of brain catecholamine, indoleamine and amino acid transmitter levels (Moore). This unit will provide logistical support and technical expertise to projects of the Center that employ in vivo microdialysis in rodents and primates (Projects by Laurelle, Kandel, Rayport) including experimental design, implementation, and chromatographic analysis of catecholamines, indolamines and amino acids in in vivo brain dialysate samples and ex vivo brain tissue homogenates (Projects by Laurelle, Javitt, Kandel, Rayport). 2) Pharmacokinetic Unit (Cooper). This unit will develop and perform analysis of plasma levels of pharmacological agents (e.g. MK 801, NNC 112, SCH 39166, amphetamine, PCP, risperidone) used in mice (Projects by Kandel, Rayport ); non-human primates (Projects by Laurelle and Project by Javitt) and humans (Projects by Abi-Dargham and Laurelle). 3) Receptor Binding Unit (Javitch). This unit will integrate activiiies performed in the laboratories of Javitch, Rayport, and Huang. The first mission is to perform in vitro binding assay and to characterize candidate radioligands developed by the Brain Imaging Core and Projects by Abi-Dargham and Project by Laurelle. The second mission is to study the impact of receptor trafficking on binding affinities of D2 and D1 radiotracers. The latter investigation will provide important information for imaging studies using endogenous competition techniques such as used in Project by Laurelle, and will contribute to the development of novel radioligands with binding that is sensitive to changes in synaptic/extracellular concentrations of endogenous transmitters, specifically dopamine and glutamate (see Brain Imaging Core). In summary, the Neurochemistry Core will share personnel and will work closely with Projects by Abi-Dargham, Laurelle, Javitt, Kandel & Rayport and the Brain Imaging Core and Cellular/Molecular Core to provide screening and hypothesis-directed characterization of neurotransmitter profiles in animal models, development of novel methods for combining in vivo microdialysis and functional brain imaging in primates and rodents, and development of methods for characterization of candidate radioligands for functional brain imaging.

神经化学核心将整合和巩固几个基本的活动 该中心的实验室分为三个单位，在摩尔博士、库珀博士和贾维奇博士的指导下， 分别： 1) 体内和离体脑儿茶酚胺、吲哚胺和氨基酸的测定 发射机电平（摩尔）。该部门将为该中心在啮齿动物和灵长类动物体内采用微透析的项目（Laurelle、Kandel、Rayport 的项目）提供后勤支持和技术专业知识，包括儿茶酚胺、吲哚胺和氨基酸的实验设计、实施和色谱分析。体内脑透析液样本和离体脑组织匀浆（Laurelle、Javitt、Kandel、Rayport 的项目）。 2) 药代动力学单位（库珀）。该单位将开发并分析血浆水平 用于小鼠的药物（例如 MK 801、NNC 112、SCH 39166、安非他明、PCP、利培酮）（Kandel 项目、Rayport 项目）；非人类灵长类动物（Laurelle 的项目和 Javitt 的项目）和人类（Abi-Dargham 和 Laurelle 的项目）。 3) 受体结合单元(Javitch)。该单元将整合在实验室进行的活动 贾维奇、雷波特和黄。第一个任务是进行体外结合测定并表征由脑成像核心和 Abi-Dargham 项目以及 Laurelle 项目开发的候选放射性配体。 第二个任务是研究受体贩运对 D2 和 D1 放射性示踪剂结合亲和力的影响。后一项研究将为使用内源竞争技术（例如 Laurelle 项目中使用的技术）的成像研究提供重要信息，并将有助于新型放射性配体的开发 对内源性递质（特别是多巴胺和谷氨酸）的突触/细胞外浓度变化敏感的结合（参见脑成像核心）。 总之，神经化学核心将共享人员，并将与 Abi-Dargham、Laurelle、Javitt、Kandel 和 Rayport 的项目以及脑成像核心和细胞/分子核心密切合作，提供动物神经递质谱的筛选和假设导向表征模型、开发将灵长类动物和啮齿动物体内微透析和功能性脑成像相结合的新方法，以及开发用于功能性脑成像的候选放射性配体的表征方法。