Role of Endocytosis in Notch and Wingless Signaling

内吞作用在 Notch 和 Wingless 信号传导中的作用

基本信息

批准号：
6861011
负责人：
Elaine Seto
金额：
$ 2.34万
依托单位：
BAYLOR COLLEGE OF MEDICINE
依托单位国家：
美国
项目类别：
财政年份：
2002
资助国家：
美国
起止时间：
2002-03-01 至 2006-02-24
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant) The goal of this research proposal is to understand the role of various endocytic trafficking events in the regulation of Notch and Wingless signaling. Focusing on the Drosophila wing, where the pathways interact, I have performed preliminary studies using Hrs, a protein implicated in multivesicular body formation. I have found genetic interactions with signaling members, as well as changes in protein levels and localization, suggestive of a role in signal regulation. Here, I plan to tease apart endocytosis by analyzing the importance of each step of trafficking on Notch and Wingless signaling regulation. I will perform a detailed study of the subcellular localization of signaling members by microscopy. Then, using genetic tools that modulate vesicle trafficking events, I will characterize changes in protein subcellular localization and analyze the resultant wing phenotypes. Finally, I will investigate the role of endocytosis in establishing the Wingless morphogen gradient. Together, these studies will contribute to our understanding of Notch and Wingless signaling and provide insight into how these pathways control cell proliferation and cell fate determination.

描述（由申请人提供）该研究建议的目的是了解各种内吞交易事件在调节和无翼信号的调节中。专注于果蝇翼，路径相互作用，我进行了使用HRS的初步研究，该蛋白质与多囊体有关形成。我发现与信号构件以及蛋白质水平和定位的变化，表明在信号中作用规定。在这里，我计划通过分析重要性来逗弄内吞作用贩运档位和无翼信号调节的每个步骤。我会对信号构件的亚细胞定位进行详细研究通过显微镜。然后，使用调节囊泡运输的遗传工具事件，我将表征蛋白质亚细胞定位的变化和分析所得的机翼表型。最后，我将调查内吞作用在建立无翅的形态梯度方面。在一起，这些研究将有助于我们对缺口和无机信号的理解并提供有关这些途径如何控制细胞增殖和细胞的洞察力命运确定。