Streptococcus-Candida Communication in Oral Biofilms

口腔生物膜中的链球菌-念珠菌通讯

• 批准号: 7142096
• 负责人: HOWARD F JENKINSON
• 金额: $ 38.66万
• 依托单位: UNIVERSITY OF BRISTOL
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-17 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7142096
• 关键词: Candida; Streptococcus; adhesin; adhesions; biofilm; clinical research; communication; receptor; yeasts

DESCRIPTION (provided by applicant): Dental plaque biofilms are polymicrobial communities found on oral surfaces embedded in a matrix of host salivary components and microbial extracellular products. These biofilms provide an infection source for diseases of the oral hard and soft tissues. Communication reactions between the micro-organisms modulate the structure, composition and pathogenic potential of the biofilms. The oral streptococci are especially important in this respect because they are primary colonizers of oral cavity surfaces. Their deposition provides an attachment substrate for colonization by potentially pathogenic organisms such as the fungus Candida albicans, which is the cause of most yeast infections in humans. Co-adhesion between oral streptococci and C. albicans is proposed to facilitate oral carriage and persistence of C. albicans, leading to disease conditions such as denture-induced stomatitis. Studies have demonstrated that inter-microbial binding of Streptococcus gordonii and C. albicans involves complementary and co-operative adhesin- receptor molecules. Streptococcal cell surface-associated antigen I/II family adhesins, designated SspA and SspB, target yeast cell surface receptors, resulting in close engagement of other surface molecules on the partner cell types. These adhesion processes drive the development and accumulation of mixed species biofilms that are morphologically, physiologically and pathogenically unique. The objectives of this application are to: identify the C. albicans receptor for the SspB protein of S. gordonii; define the molecular basis of yeast receptor recognition by antigen l/ll family proteins; investigate the effects of substratum composition, environmental factors and the role of Streptococcal adhesin expression on the development of S. gordonii-C. albicans mixed biofilms; determine patterns of differential gene expression in S. gordonii and C. albicans in biofilms and induced by cell-cell contact. These experiments will provide detailed molecular information on the major components mediating interactions of S. gordonii and C. albicans and better understanding of the signals and pathways by which co-adhesion leads to biofilm maturation. Such information will assist in the development of more effective biologically based strategies for control of polymicrobial biofilms, and for therapeutic prevention of C. albicans overgrowth. Candida yeast infections are a serious threat to public health. This research devises new methods for controlling or preventing growth of Candida in the mouth.

描述（由申请人提供）：牙菌斑生物膜是在嵌入宿主唾液成分和微生物细胞外产物基质中的口腔表面上发现的多数菌群群落。这些生物膜为口腔硬组织和软组织疾病提供了感染来源。微生物之间的通信反应调节生物膜的结构，组成和致病潜力。口服链球菌在这方面尤其重要，因为它们是口腔表面的主要菌落。它们的沉积为潜在的致病生物（例如真菌白色念珠菌）提供了固定的附着底物，这是人类大多数酵母菌感染的原因。提出了口服链球菌和白色念珠菌之间的共粘附，以促进白色念珠菌的口腔运输和持久性，从而导致疾病，例如牙齿诱发的口腔炎。研究表明，Gordonii链球菌和白色念珠菌的微生物间结合涉及互补和合作性粘附素受体分子。链球菌细胞表面相关的抗原I/II家族粘附素，指定的SSPA和SSPB，靶酵母细胞表面受体，导致其他表面分子在伴侣细胞类型上紧密接合。这些粘附过程推动了混合物种生物膜的发展和积累，这些物种在形态，生理和病理上是独特的。该应用的目标是：识别S.Gordonii的SSPB蛋白的白色念珠菌受体；定义抗原L/LL家族蛋白识别酵母受体识别的分子基础；研究了基质组成，环境因素以及链球菌粘附素表达对戈尔多尼链球菌发展的作用的影响。白色唱片混合生物膜；确定生物膜中Gordonii和白色念珠菌中差异基因表达的模式，并通过细胞 - 细胞接触诱导。这些实验将提供有关介导Gordonii和白色念珠菌相互作用的主要组成部分的详细分子信息，并更好地理解共粘附导致生物膜成熟的信号和途径。这些信息将有助于制定更有效的基于生物学的策略，以控制多菌生物膜，并预防白色念珠菌过度的治疗性预防。念珠菌酵母菌感染是对公共卫生的严重威胁。这项研究为控制或防止口腔中念珠菌的生长设计了新的方法。