Optical Biopsy sing Optical Coherence Tomography

光学相干断层扫描光学活检

• 批准号: 6941394
• 负责人: JAMES G FUJIMOTO
• 金额: $ 24.1万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-05 至 2008-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6941394
• 关键词: Barretts esophagus; adenocarcinoma; bioimaging /biomedical imaging; biomedical equipment development; biopsy; clinical research; colon neoplasms; confocal scanning microscopy; diagnosis design /evaluation; disease /disorder model; early diagnosis; fiber optic microscopy; gastrointestinal system; hamsters; human subject; hyperplasia; imaging /visualization /scanning; laboratory rabbit; lasers; neoplasm /cancer diagnosis; optical tomography; respiratory system; squamous cell carcinoma

DESCRIPTION (provided by applicant): The hypothesis of this proposal is that optical coherence tomography (OCT), an emerging biomedical! diagnostic imaging technology for in situ imaging of tissue microstructure, can be developed and applied for "optical biopsy," the real time, in vivo detection of early neoplastic changes. Image guidance can be coupled with excisional biopsy to reduce sampling error and improve sensitivity. This program integrates new technology development with applications to biomedical and clinical studies. The specific aims are: 1. Develop ultrahigh resolution OCT and spectroscopic OCT technology, improving the current 10-15 um resolution to the 1-2 um level. This dramatic improvement in image resolution will significantly enhance the performance of OCT for imaging of architectural morphology and will facilitate the identification of early neoplastic changes such as dysplasia. Spectroscopic OCT imaging will improve differentiation of tissue based on spectroscopic properties and promises to provide assessment of cellular morphology. These studies will utilize state of the art laser technology developed in our group, enabling ultrahigh resolution imaging in broad wavelength regimes to optimize image penetration and resolution. 2. Develop new optical coherence microscopy (OCM) technology, which integrates OCT with confocal microscopy. OCM enables cellular level resolution and has image penetration depths greater than confocal microscopy. It also performs imaging without the need for high numerical apertures required in confocal microscopy, thus enabling imaging with small probes such as laparoscopes, endoscopes, and needles. 3. Investigate OCT imaging in normal animals and models of cancer progression. Imaging studies will be performed in the normal rabbit to validate new endoscopic imaging techniques. The hamster cheek pouch model of squamous cell carcinoma and the AOM rat model of colon cancer will be used to image cancer progression. 4. Investigate ultrahigh resolution OCT and spectroscopic OCT imaging of the GI tract. This aim will involve imaging of ex vivo specimens and endoscopic imaging studies. The feasibility of OCT imaging to differentiate short segment Barrett's esophagus, dysplasia and adenocarcinoma of the esophagus, and adenomatous vs. hyperplastic polyps in the colon will be explored. This program is a multidisciplinary collaborative effort with investigators at the VA Boston Healthcare system. If successful, this research will develop new imaging technology with applications in gastrointestinal endoscopy as well as the detection of a wide range of early neoplastic changes.

描述（由申请人提供）： 该提议的假设是光学相干断层扫描（OCT），一种新兴的生物医学！可以开发并应用用于“光学活检”的实时成像的诊断成像技术，即实时，在体内检测早期肿瘤变化。图像引导可以与移动活检结合起来，以减少采样误差并提高灵敏度。该计划将新技术开发与生物医学和临床研究的应用相结合。具体目的是：1。开发超高分辨率OCT和光谱OCT技术，将目前的10-15 UM分辨率提高到1-2 UM水平。图像分辨率的这种显着改善将显着提高OCT对建筑形态的成像的性能，并将促进早期肿瘤变化（如发育异常）的鉴定。光谱OCT成像将根据光谱特性改善组织的分化，并有望提供细胞形态的评估。这些研究将利用我们小组中开发的最先进的激光技术，从而在广泛的波长状态下实现超高分辨率成像，以优化图像渗透和分辨率。 2。开发新的光学相干显微镜（OCM）技术，该技术将OCT与共聚焦显微镜集成。 OCM可以实现细胞水平分辨率，并具有大于共聚焦显微镜的图像穿透深度。它还执行成像，而无需共聚焦显微镜中需要的高数值孔径，从而可以使用小探针（例如腹腔镜，内窥镜和针头）进行成像。 3。研究正常动物和癌症进展模型中的OCT成像。成像研究将在正常的兔子中进行，以验证新的内窥镜成像技术。鳞状细胞癌的仓鼠脸颊袋模型和结肠癌的AOM大鼠模型将用于对癌症的进展进行图像。 4。研究GI道的超高分辨率OCT和光谱OCT成像。此目标将涉及对离体标本和内窥镜成像研究的成像。将探索OCT成像区分短片段Barrett的食管，发育异常和腺癌的可行性，以及结肠中的腺瘤与增生性息肉的可行性。该计划是与VA波士顿医疗保健系统的调查人员进行的多学科合作工作。如果成功，这项研究将开发新的成像技术，并在胃肠道内窥镜检查中应用以及检测广泛的早期肿瘤变化。